High Resolution of Threonine Protein Kinase
Excessive decision two-dimensional electrophoresis of proteins
Proteins are separated in response to isoelectric level by isoelectric focusing within the first dimension, and in response to molecular weight by sodium dodecyl sulfate electrophoresis within the second dimension. Since these two parameters are unrelated, it’s potential to acquire an virtually uniform distribution of protein spots throughout a two-diminsional gel.
Serine/Threonine-Protein Kinase N1 (PKN1) Antibody |
This system has resolved 1100 completely different elements from Escherichia coli and needs to be able to resolving a most of 5000 proteins. A protein containing as little as one disintegration per min of both 14C or 35S might be detected by autoradiography.
A protein which constitutes 10 minus four to 10 minus 5% of the entire protein might be detected and quantified by autoradiography. The reproducibility of the separation is enough to allow every spot on one separation to be matched with a spot on a special separation.
This system offers a technique for estimation (on the described sensitivities) of the variety of proteins made by any organic system. This method can resolve proteins differing in a single cost and consequently can be utilized within the evaluation of in vivo modifications leading to a change in cost. Proteins whose cost is modified by missense mutations might be recognized. An in depth description of the strategies in addition to the traits of this method are offered.

stat-2
Recombinant EBV EBNA1 Protein, His, E.coli-100ug |
Recurrent urinary tract infections (rUTI) are a crucial sickness associated to morbidities and mortality. Resistance to the same old of care antibiotics is now widespread as a result of continued use of antibiotics amongst people who endure from rUTI.
- We’re subsequently making a vaccine to forestall recurrences amongst victims with rUTI. The antigen of the vaccine is FimH, a bacterial adhesin protein, and the vaccine is adjuvanted with a TLR-4 agonist.
- In a Part 1 scientific look at evaluating the vaccine, immunized individuals produced FimH-binding antibodies. Proper right here we describe the optimization, qualification, and use of an assay to judge the efficiency of these anti-FimH antibodies.
- The suitability of the assay for its supposed perform was demonstrated by selectivity, specificity, sensitivity, and intra-assay and inter-assay precision.
- The acceptance requirements have been achieved for all parameters along with intra-assay precision with ≤10% relative regular deviations and inter-assay precision with ≤25% relative regular deviations. The outcomes provided herein advocate this handy assay will most likely be needed for supporting the vaccine’s efficacy in future human analysis.
- Furthermore and of good significance, these outcomes present that vaccine-induced helpful antibodies could also be elicited in rUTI victims in the direction of an important virulence situation, FimH.
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YF-PA13997 | Abfrontier | 50 ug | EUR 363 |
Description: Mouse polyclonal to PKN1 |
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YF-PA13998 | Abfrontier | 100 ug | EUR 403 |
Description: Rabbit polyclonal to PKN1 |
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YF-PA27338 | Abfrontier | 100 ul | EUR 403 |
Description: Rabbit polyclonal to PKN1 |
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RD-PKN1-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 478 |
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RD-PKN1-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 662 |
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DLR-PKN1-Hu-48T | DL Develop | 48T | EUR 479 |
Description: A sandwich quantitative ELISA assay kit for detection of Human Protein Kinase N1 (PKN1) in samples from tissue homogenates, cell lysates or other biological fluids. |
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DLR-PKN1-Hu-96T | DL Develop | 96T | EUR 621 |
Description: A sandwich quantitative ELISA assay kit for detection of Human Protein Kinase N1 (PKN1) in samples from tissue homogenates, cell lysates or other biological fluids. |
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RDR-PKN1-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 500 |
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RDR-PKN1-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 692 |
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Anti-CRBN | ImmunoStep | 50 µg | EUR 349 |
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YF-MA14890 | Abfrontier | 100 ug | EUR 363 |
Description: Mouse monoclonal to PKN1 |
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YF-MA14891 | Abfrontier | 100 ug | EUR 363 |
Description: Mouse monoclonal to PKN1 |
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1-CSB-PA030259 | Cusabio |
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Description: A polyclonal antibody against PKN1. Recognizes PKN1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000 |
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48392-100ul | SAB | 100ul | EUR 333 |
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48392-50ul | SAB | 50ul | EUR 239 |
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DF4790 | Affbiotech | 200ul | EUR 304 |
Description: PKN1 Antibody detects endogenous levels of total PKN1. |
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ABD4790 | Lifescience Market | 100 ug | EUR 438 |
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70R-50256 | Fitzgerald | 100 ul | EUR 244 |
Description: Purified Polyclonal PKN1 antibody |
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ES8989-100ul | ELK Biotech | 100ul | EUR 279 |
Description: A Rabbit Polyclonal antibody against PKN1 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA |
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ES8989-50ul | ELK Biotech | 50ul | EUR 207 |
Description: A Rabbit Polyclonal antibody against PKN1 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA |
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35295-100ul | SAB | 100ul | EUR 252 |
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35295-50ul | SAB | 50ul | EUR 187 |
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ABP59926-003ml | Abbkine | 0.03ml | EUR 158 |
Description: A polyclonal antibody for detection of PKN1 from Human, Mouse, Rat. This PKN1 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PKN1 protein at amino acid sequence of 720-800 |
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ABP59926-01ml | Abbkine | 0.1ml | EUR 289 |
Description: A polyclonal antibody for detection of PKN1 from Human, Mouse, Rat. This PKN1 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PKN1 protein at amino acid sequence of 720-800 |
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ABP59926-02ml | Abbkine | 0.2ml | EUR 414 |
Description: A polyclonal antibody for detection of PKN1 from Human, Mouse, Rat. This PKN1 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PKN1 protein at amino acid sequence of 720-800 |
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C48392 | SAB | 100ul | EUR 397 |
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ELI-37289r | Lifescience Market | 96 Tests | EUR 886 |
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20-abx928745 | Abbexa |
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20-abx928746 | Abbexa |
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20-abx904045 | Abbexa |
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C35295 | SAB | 100ul | EUR 397 |
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GST-ANTI-1 | Detroit R&D | 50 uL | EUR 280 |
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GST-ANTI-2 | Detroit R&D | 50 uL | EUR 280 |
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GST-ANTI-3 | Detroit R&D | 50 uL | EUR 280 |
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CSB-CL623082HU-10ug | Cusabio | 10ug | EUR 902 |
Description: A cloning plasmid for the PKN1 gene. |
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A0553-100ul | Abclonal | 100 ul | EUR 308 |
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A0553-200ul | Abclonal | 200 ul | EUR 459 |
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A0553-20ul | Abclonal | 20 ul | EUR 183 |
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A0553-50ul | Abclonal | 50 ul | EUR 223 |
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20-abx063131 | Abbexa |
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DF4790-BP | Affbiotech | 1mg | EUR 195 |
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SPC-1065D | Stressmarq | 0.1ml | EUR 354 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is unconjugated. |
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SPC-1065D-A390 | Stressmarq | 0.1ml | EUR 401 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is conjugated to ATTO 390. |
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SPC-1065D-A488 | Stressmarq | 0.1ml | EUR 400 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is conjugated to ATTO 488. |
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SPC-1065D-A565 | Stressmarq | 0.1ml | EUR 400 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is conjugated to ATTO 565. |
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SPC-1065D-A594 | Stressmarq | 0.1ml | EUR 400 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is conjugated to ATTO 594. |
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SPC-1065D-A633 | Stressmarq | 0.1ml | EUR 400 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is conjugated to ATTO 633. |
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SPC-1065D-A655 | Stressmarq | 0.1ml | EUR 400 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is conjugated to ATTO 655. |
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SPC-1065D-A680 | Stressmarq | 0.1ml | EUR 400 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is conjugated to ATTO 680. |
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SPC-1065D-A700 | Stressmarq | 0.1ml | EUR 400 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is conjugated to ATTO 700. |
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SPC-1065D-ALP | Stressmarq | 0.1ml | EUR 394 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is conjugated to Alkaline Phosphatase. |
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SPC-1065D-APC | Stressmarq | 0.1ml | EUR 399 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is conjugated to APC . |
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SPC-1065D-APCCY7 | Stressmarq | 0.1ml | EUR 471 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is conjugated to APC/Cy7. |
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SPC-1065D-BI | Stressmarq | 0.1ml | EUR 396 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is conjugated to Biotin. |
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SPC-1065D-DY350 | Stressmarq | 0.1ml | EUR 475 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is conjugated to Dylight 350. |
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SPC-1065D-DY405 | Stressmarq | 0.1ml | EUR 452 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is conjugated to Dylight 405. |
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SPC-1065D-DY488 | Stressmarq | 0.1ml | EUR 432 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is conjugated to Dylight 488. |
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SPC-1065D-DY594 | Stressmarq | 0.1ml | EUR 436 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is conjugated to Dylight 594. |
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SPC-1065D-DY633 | Stressmarq | 0.1ml | EUR 426 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is conjugated to Dylight 633. |
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SPC-1065D-FITC | Stressmarq | 0.1ml | EUR 392 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is conjugated to FITC. |
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SPC-1065D-HRP | Stressmarq | 0.1ml | EUR 388 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is conjugated to HRP. |
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SPC-1065D-P594 | Stressmarq | 0.1ml | EUR 407 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is conjugated to PE/ATTO 594. |
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SPC-1065D-PCP | Stressmarq | 0.1ml | EUR 399 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is conjugated to PerCP. |
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SPC-1065D-RPE | Stressmarq | 0.1ml | EUR 397 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is conjugated to RPE . |
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SPC-1065D-STR | Stressmarq | 0.1ml | EUR 398 |
Description: A polyclonal antibody for PKN1 (pThr774) from Human. The antibody is produced in rabbit after immunization with Human A phospho-specific peptide corresponding to residues surrounding Thr774 of human PKN1 (AA771-777). The Antibody is tested and validated for WB, AM assays with the following recommended dilutions: WB (1:250). This PKN1 (pThr774) antibody is conjugated to Streptavidin. |
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AMM07214G | Leading Biology | 0.1ml | EUR 528 |
Description: A Monoclonal antibody against Human PKN1. The antibodies are raised in Rabbit and are from clone EPR3238. This antibody is applicable in WB, FC |
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AMM03009G | Leading Biology | 0.1ml | EUR 528 |
Description: A Monoclonal antibody against Human PKN1. The antibodies are raised in Mouse and are from clone 4H10B1. This antibody is applicable in WB and IHC, FC, ICC, E |
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20-abx101658 | Abbexa |
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20-abx135704 | Abbexa |
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20-abx008827 | Abbexa |
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20-abx174267 | Abbexa |
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EAP0125 | Abclonal | 96Tests | EUR 521 |
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ERP0125 | Abclonal | 96Tests | EUR 521 |
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ERTP0125 | Abclonal | 96Tests | EUR 521 |
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EMP0125 | Abclonal | 96Tests | EUR 521 |
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EPP0125 | Abclonal | 96Tests | EUR 521 |
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EHP0125 | Abclonal | 96Tests | EUR 521 |
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ECP0125 | Abclonal | 96Tests | EUR 521 |
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EBP0125 | Abclonal | 96Tests | EUR 521 |
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EGTP0125 | Abclonal | 96Tests | EUR 521 |
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20-abx983453 | Abbexa |
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20-abx985442 | Abbexa |
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20-abx953755 | Abbexa |
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EF005461 | Lifescience Market | 96 Tests | EUR 689 |
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4-PAA501Hu01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Protein Kinase N1 (PKN1) |
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abx149738-100ug | Abbexa | 100 ug | EUR 439 |
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20-abx015170 | Abbexa |
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abx224112-100ug | Abbexa | 100 ug | EUR 411 |
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20-abx327640 | Abbexa |
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4-RPA501Hu01 | Cloud-Clone |
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Description: Recombinant Human Protein Kinase N1 expressed in: E.coli |
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EGP0125 | Abclonal | 96Tests | EUR 521 |
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OKCD00803 | Aviva Systems Biology | 96 Wells | EUR 792 |
Description: Description of target: PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.11 Publications
<p>Manually curated information for which there is published experimental evidence.</p>
<p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.7"PRK1 phosphorylates MARCKS at the PKC sites: serine 152, serine 156 and serine 163."_x005F_x005F_x000D_Palmer R.H., Schonwasser D.C., Rahman D., Pappin D.J., Herget T., Parker P.J._x005F_x005F_x000D_FEBS Lett. 378:281-285(1996) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.Ref.8"PKN associates and phosphorylates the head-rod domain of neurofilament protein."_x005F_x005F_x000D_Mukai H., Toshimori M., Shibata H., Kitagawa M., Shimakawa M., Miyahara M., Sunakawa H., Ono Y._x005F_x005F_x000D_J. Biol. Chem. 271:9816-9822(1996) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.Ref.10"Domain-specific phosphorylation of vimentin and glial fibrillary acidic protein by PKN."_x005F_x005F_x000D_Matsuzawa K., Kosako H., Inagaki N., Shibata H., Mukai H., Ono Y., Amano M., Kaibuchi K., Matsuura Y., Azuma I., Inagaki M._x005F_x005F_x000D_Biochem. Biophys. Res. Commun. 234:621-625(1997) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.Ref.14"Phosphorylation of tau is regulated by PKN."_x005F_x005F_x000D_Taniguchi T., Kawamata T., Mukai H., Hasegawa H., Isagawa T., Yasuda M., Hashimoto T., Terashima A., Nakai M., Mori H., Ono Y., Tanaka C._x005F_x005F_x000D_J. Biol. Chem. 276:10025-10031(2001) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.Ref.15"A novel inducible transactivation domain in the androgen receptor: implications for PRK in prostate cancer."_x005F_x005F_x000D_Metzger E., Muller J.M., Ferrari S., Buettner R., Schule R._x005F_x005F_x000D_EMBO J. 22:270-280(2003) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ANDR.Ref.19"Rho GTPases regulate PRK2/PKN2 to control entry into mitosis and exit from cytokinesis."_x005F_x005F_x000D_Schmidt A., Durgan J., Magalhaes A., Hall A._x005F_x005F_x000D_EMBO J. 26:1624-1636(2007) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, PHOSPHORYLATION, SUBCELLULAR LOCATION.Ref.22"Phosphorylation of histone H3 at threonine 11 establishes a novel chromatin mark for transcriptional regulation."_x005F_x005F_x000D_Metzger E., Yin N., Wissmann M., Kunowska N., Fischer K., Friedrichs N., Patnaik D., Higgins J.M., Potier N., Scheidtmann K.H., Buettner R., Schule R._x005F_x005F_x000D_Nat. Cell Biol. 10:53-60(2008) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF LYS-644.Ref.27"Protein kinase C-related kinase targets nuclear localization signals in a subset of class IIa histone deacetylases."_x005F_x005F_x000D_Harrison B.C., Huynh K., Lundgaard G.L., Helmke S.M., Perryman M.B., McKinsey T.A._x005F_x005F_x000D_FEBS Lett. 584:1103-1110(2010) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.Ref.31"A-kinase anchoring protein (AKAP)-Lbc anchors a PKN-based signaling complex involved in alpha1-adrenergic receptor-induced p38 activation."_x005F_x005F_x000D_Cariolato L., Cavin S., Diviani D._x005F_x005F_x000D_J. Biol. Chem. 286:7925-7937(2011) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, IDENTIFICATION IN A COMPLEX WITH AKAP13; MAPK14; ZAK AND MAP2K3.Ref.32"Regulatory domain selectivity in the cell-type specific PKN-dependence of cell migration."_x005F_x005F_x000D_Lachmann S., Jevons A., De Rycker M., Casamassima A., Radtke S., Collazos A., Parker P.J._x005F_x005F_x000D_PLoS ONE 6:E21732-E21732(2011) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN CELL MIGRATION, TISSUE SPECIFICITY.Ref.41"Structure of an SspH1-PKN1 complex reveals the basis for host substrate recognition and mechanism of activation for a bacterial E3 ubiquitin ligase."_x005F_x005F_x000D_Keszei A.F., Tang X., McCormick C., Zeqiraj E., Rohde J.R., Tyers M., Sicheri F._x005F_x005F_x000D_Mol. Cell. Biol. 34:362-373(2014) [PubMed] [Europe PMC] [Abstract]Cited for: X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS) OF 122-199 IN COMPLEX WITH SALMONELLA SSPH1, UBIQUITINATION (MICROBIAL INFECTION), MUTAGENESIS OF ARG-181 AND ARG-185, FUNCTION AS ANDROGEN RECEPTOR COACTIVATOR, INTERACTION WITH SALMONELLA SSPH1 (MICROBIAL INFECTION). ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.117 ng/mL |
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OKEI00529 | Aviva Systems Biology | 96 Wells | EUR 767 |
Description: Description of target: PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.938 ng/mL |
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OKEI00825 | Aviva Systems Biology | 96 Wells | EUR 767 |
Description: Description of target: PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation . Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.;Species reactivity: Rat;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.938 ng/mL |
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ORF073569 | ABM | 1.0 ug DNA | EUR 506 |
The influenza A virus (IAV) matrix-2 (M2) protein is an antigenically conserved viral envelope protein that performs an needed place in virus budding together with one different envelope protein, hemagglutinin (HA). An M2-specific mouse monoclonal IgG antibody, rM2ss23, which binds to the ectodomain of the M2 protein, has been confirmed to be a non-neutralizing antibody, nevertheless inhibits plaque formation of IAV strains.
EBNA1 Binding Protein 2 (EBNA1BP2) Antibody |
On this look at, we generated chimeric rM2ss23 (ch-rM2ss23) IgG and IgA antibodies with the similar variable space and in distinction their antiviral actions. Using gel chromatography, ch-rM2ss23 IgA have been divided into three antibody subsets: monomeric IgA (m-IgA), dimeric IgA (d-IgA), and trimeric and tetrameric IgA (t/q-IgA). We found that t/q-IgA had a significantly better functionality to chop again the plaque measurement of IAVs than IgG and m-IgA, virtually actually on account of decreased number of progeny virus particles produced from contaminated cells. Apparently, HA-M2 colocalization was remarkably decreased on the contaminated cell ground throughout the presence of ch-rM2ss23 antibodies.
These outcomes level out that anti-M2 polymeric IgA restricts IAV budding additional successfully than IgG and advocate a job of anti-M2 IgA in cross-protective immunity to IAVs.
- Children with Coronavirus Sickness 2019 (COVID-19) have been reported to point milder indicators and better prognosis than their grownup counterparts, nevertheless the excellence of immune response in the direction of SARS-CoV-2 between children and adults hasn’t been reported.Attributable to this truth we initiated this look at to work out the choices of immune response in children with COVID-19. Sera and full blood cells from 19 children with COVID-19 all through completely completely different phases after sickness onset have been collected.
- The cytokine concentrations, SARS-CoV-2 S-RBD or N-specific antibodies and T cell immune responses have been detected respectively. In children with COVID-19, solely three of 12 cytokines have been elevated in acute sera, along with interferon (IFN)-γ-induced protein 10 (IP10), interleukin (IL)-10 and IL-16. We seen an increase in T helper (Th)-2 cells and a suppression in regulatory T cells (Treg) in victims all through acute part, nevertheless no very important response was found throughout the IFN-γ-producing or tumor necrosis situation (TNF)-α-producing CD8+ T cells in victims.
- S-RBD and N IgM confirmed an early induction, whereas S-RBD and N IgG have been prominently induced later in convalescent part. Potent S-RBD IgA response was seen nevertheless N IgA seemed to be inconspicuous.
![]() EBV EBNA1 antibody |
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20-EG46 | Fitzgerald | 1 mg | EUR 116 |
Description: Goat polyclonal EBV EBNA1 antibody |
![]() Anti-EBV-EBNA1 antibody |
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STJ16101052 | St John's Laboratory | 100 µg | EUR 354 |
![]() EBV Mosaic EBNA1 protein [GST] |
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DAG1577 | Creative Diagnostics | 100 µg | EUR 645 |
![]() Recombinant EBV EBNA1 Protein, His, E.coli-100ug |
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QP11731-100ug | EnQuireBio | 100ug | EUR 318 |
![]() Recombinant EBV EBNA1 Protein, His, E.coli-1mg |
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QP11731-1mg | EnQuireBio | 1mg | EUR 1561 |
![]() Recombinant EBV EBNA1 Protein, His, E.coli-500ug |
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QP11731-500ug | EnQuireBio | 500ug | EUR 862 |
![]() Recombinant EBV EBNA1 Mosaic Protein, His, E.coli-100ug |
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QP11732-100ug | EnQuireBio | 100ug | EUR 218 |
![]() Recombinant EBV EBNA1 Mosaic Protein, His, E.coli-1mg |
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QP11732-1mg | EnQuireBio | 1mg | EUR 1061 |
![]() Recombinant EBV EBNA1 Mosaic Protein, His, E.coli-500ug |
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QP11732-500ug | EnQuireBio | 500ug | EUR 663 |
![]() EBNA1 protein |
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30-1925 | Fitzgerald | 500 ug | EUR 565 |
Description: Purified Recombinant EBNA1 protein |
![]() Recombinant EBV EBNA1 Mosaic Protein (aa 1-90 & 408-498) [His] |
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VAng-2528Lsx-100g | Creative Biolabs | 100 µg | EUR 463 |
Description: EBV EBNA1 Mosaic protein, His tag at C-terminus, recombinant protein from E. coli. |
![]() Recombinant EBV EBNA1 Mosaic Protein (aa 1-90 & 408-498) [His] |
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VAng-2528Lsx-1mg | Creative Biolabs | 1 mg | EUR 2800 |
Description: EBV EBNA1 Mosaic protein, His tag at C-terminus, recombinant protein from E. coli. |
![]() Recombinant EBV EBNA1 Mosaic Protein (aa 1-90 & 408-498) [His] |
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VAng-2528Lsx-500g | Creative Biolabs | 500 µg | EUR 1700 |
Description: EBV EBNA1 Mosaic protein, His tag at C-terminus, recombinant protein from E. coli. |
![]() Recombinant EBV EBNA1 Mosaic Protein (aa 1-90 & 408-498) [GST] |
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VAng-Lsx0111-100g | Creative Biolabs | 100 µg | EUR 633 |
Description: EBV EBNA1 Mosaic protein [GST], recombinant protein from E. coli. |
![]() Recombinant EBV EBNA1 Mosaic Protein (aa 1-90 & 408-498) [GST] |
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VAng-Lsx0111-500g | Creative Biolabs | 500 µg | EUR 2016 |
Description: EBV EBNA1 Mosaic protein [GST], recombinant protein from E. coli. |
![]() pCXWB- EBNA1 |
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PVT10196 | Lifescience Market | 2 ug | EUR 301 |
![]() Recombinant (E.Coli, GST tag) Epstein-Barr Virus (EBV/HHV-4) Mosaic EBNA1 |
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RP-347 | Alpha Diagnostics | 100 ug | EUR 286 |
![]() Recombinant EBNA1 Protein [GST] |
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VAng-Lsx0122-100g | Creative Biolabs | 100 µg | EUR 682 |
Description: EBNA1 (EBV) (P03211) partial recombinant protein with GST tag expressed in E. coli. |
![]() EBNA1 protein (His tag) |
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80-1340 | Fitzgerald | 100 ug | EUR 241 |
Description: Purified recombinant EBNA1 protein (His tag) |
![]() EBV Protein |
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abx069822-1ml | Abbexa | 1 ml | EUR 690 |
![]() EBV Protein |
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abx069824-1mg | Abbexa | 1 mg | EUR 1469 |
![]() EBV Protein |
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abx069825-1ml | Abbexa | 1 ml | EUR 314 |
![]() EBV Protein |
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abx069828-1mg | Abbexa | 1 mg | EUR 1817 |
![]() EBV Protein |
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abx069829-1mg | Abbexa | 1 mg | EUR 1776 |
![]() EBV protein |
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30-1275 | Fitzgerald | 1 mg | EUR 3481 |
Description: Purified recombinant EBV protein |
![]() EBV protein |
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30-1276 | Fitzgerald | 1 mg | EUR 3481 |
Description: Purified recombinant EBV protein |
![]() EBNA1 Binding Protein 2 Protein |
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20-abx260778 | Abbexa |
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![]() pCXWB- EBNA1 Plasmid |
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PVT7175 | Lifescience Market | 2 ug | EUR 266 |
![]() Recombinant EBV Protein |
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VAng-Lsx0108-inquire | Creative Biolabs | inquire | Ask for price |
Description: EBV, recombinant protein from human cells. |
![]() EBV EA protein |
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30-1280 | Fitzgerald | 1 mg | EUR 3481 |
Description: Purified recombinant EBV EA protein |
![]() EBV VCA protein |
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30-1811 | Fitzgerald | 1 ml | EUR 381 |
Description: Purified native EBV VCA gp125 protein |
![]() EBV EA protein |
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30-1920 | Fitzgerald | 1 ml | EUR 414 |
Description: Viral lysate containing a high concentration of EBV antigens, including VCA, EBNA, EA-D and EA-R |
![]() EBV EBNA protein |
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30-AE46 | Fitzgerald | 200 ug | EUR 750 |
Description: Purified recombinant EBV EBNA protein |
![]() EBV EA protein |
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30-AE50 | Fitzgerald | 200 ug | EUR 813 |
Description: Purified recombinant EBV EA protein |
![]() EBV EA protein |
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30R-AE005 | Fitzgerald | 100 ug | EUR 327 |
Description: Purified recombinant EBV EA protein |
![]() EBV p18 protein |
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30R-AE007 | Fitzgerald | 500 ug | EUR 806 |
Description: Purified recombinant EBV p18 protein |
![]() EBV p23 protein |
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30R-AE008 | Fitzgerald | 100 ug | EUR 327 |
Description: Purified recombinant EBV p23 protein |
![]() EBNA1 (GFP-Puro) Lentivirus |
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LVP1134-GP | GenTarget | 1x107 IFU/ml x 200ul | EUR 349 |
Description: Premade lentivirus expressing Epstein Barr Virus' EBNA1 gene under EF1a promoter, containing GFP-Puromycin dual marker. |
![]() EBNA1 (RFP-Bsd) Lentivirus |
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LVP1134-RB | GenTarget | 1x107 IFU/ml x 200ul | EUR 349 |
Description: Premade lentivirus expressing Epstein Barr Virus' EBNA1 gene under EF1a promoter, containing RFP-Blasticidin dual marker. |
![]() EBNA1 Binding Protein 2 (EBNA1BP2) Antibody |
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20-abx123650 | Abbexa |
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![]() EBNA1 Binding Protein 2 (EBNA1BP2) Antibody |
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abx026949-400ul | Abbexa | 400 ul | EUR 523 |
![]() EBNA1 Binding Protein 2 (EBNA1BP2) Antibody |
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abx026949-80l | Abbexa | 80 µl | EUR 286 |
![]() EBV [His] |
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DAG1578 | Creative Diagnostics | 100 µg | EUR 810 |
![]() EBV [GST] |
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DAG1580 | Creative Diagnostics | 100 µg | EUR 645 |
![]() EBV [GST] |
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DAG1581 | Creative Diagnostics | 100 µg | EUR 810 |
![]() EBV [His] |
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DAG1584 | Creative Diagnostics | 100 µg | EUR 810 |
![]() EBV Virus |
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G229 | ABM | 10 ml | EUR 735 |
![]() EBV antibody |
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10-E40C | Fitzgerald | 200 ug | EUR 229 |
Description: Mouse monoclonal EBV antibody |
![]() EBV antibody |
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10-E40D | Fitzgerald | 200 ug | EUR 136 |
Description: Mouse monoclonal EBV antibody |
![]() EBV antibody |
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10-E40E | Fitzgerald | 200 ug | EUR 212 |
Description: Mouse monoclonal EBV antibody |
![]() EBV P18 Mosaic protein |
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DAG1582 | Creative Diagnostics | 100 µg | EUR 645 |
![]() EBV VCA p23 Protein |
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abx069823-1mg | Abbexa | 1 mg | EUR 1497 |
![]() Recombinant EBV Protein [His] |
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VAng-Lsx0112-inquire | Creative Biolabs | inquire | Ask for price |
Description: EBV [His], recombinant protein from E. coli. |
![]() Recombinant EBV P125 Protein |
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VAng-Lsx0119-1mL | Creative Biolabs | 1 mL | EUR 388 |
Description: EBV P125 Protein, recombinant protein from E. coli. |
![]() Recombinant EBV P125 Protein |
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VAng-Lsx0119-25mL | Creative Biolabs | 25 mL | EUR 6099 |
Description: EBV P125 Protein, recombinant protein from E. coli. |
![]() Epstein-Barr Virus (HHV-4) EBNA1 Protein |
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20-abx260340 | Abbexa |
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![]() Recombinant EBNA1 Protein [His] (1.185 mg/mL) |
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VAng-0610Lsx-inquire | Creative Biolabs | inquire | Ask for price |
Description: Epstein-Barr Virus (EBV) Nuclear Antigen-1 (EBNA-1), recombinant protein from E. coli. MW 63 kDa, 1.185 mg/mL. |
![]() Recombinant EBNA1 Protein [His] (1.25 mg/mL) |
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VAng-0611Lsx-inquire | Creative Biolabs | inquire | Ask for price |
Description: Epstein-Barr Virus (EBV) Nuclear Antigen-1 (EBNA-1), recombinant protein from E. coli. MW 63 kDa, 1.25 mg/mL. |
![]() EBNA1BP2 EBNA1 Binding Protein 2 Human Recombinant Protein |
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PROTQ99848 | BosterBio | Regular: 20ug | EUR 317 |
Description: Recombinant Human EBNA1BP2 produced in E. coli is a single polypeptide chain containing 329 amino acids (1-306) and having a molecular mass of 37.2kDa.;EBNA1BP2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
![]() EBV HHV-4 p23 Protein |
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abx060518-1mg | Abbexa | 1 mg | EUR 1887 |
![]() EBV p54 (EA-D) Protein |
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abx061433-1mg | Abbexa | 1 mg | EUR 1511 |
![]() Recombinant EBV P18 Mosaic Protein |
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VAng-Lsx0115-inquire | Creative Biolabs | inquire | Ask for price |
Description: EBV P18 Mosaic protein, recombinant protein from E. coli. |
![]() Recombinant EBV P23 Protein [His] |
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VAng-Lsx0117-inquire | Creative Biolabs | inquire | Ask for price |
Description: Recombinant EBV p23 protein was expressed in E. coli and purified by proprietary chromatographic technique, 17.7kDa. |
![]() Recombinant EBV BFRF3 Protein [GST] |
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VAng-Lsx0120-inquire | Creative Biolabs | inquire | Ask for price |
Description: BFRF3 (EBV) (P14348) partial recombinant protein with GST tag expressed in E. coli. |
![]() Recombinant EBV BMRF1 Protein [GST] |
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VAng-Lsx0121-inquire | Creative Biolabs | inquire | Ask for price |
Description: BMRF1 (EBV) (P03191) partial recombinant protein with GST tag expressed in E. coli. |
![]() Inactivated EBV gp125 VCA Protein |
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VAng-Lsx04574-1mL | Creative Biolabs | 1 mL | EUR 353 |
Description: Epstein-Barr Virus (Strain P3HR1) gp125 VCA, inactivated antigen. |
![]() Inactivated EBV gp125 VCA Protein |
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VAng-Lsx04574-25mL | Creative Biolabs | 25 mL | EUR 6099 |
Description: Epstein-Barr Virus (Strain P3HR1) gp125 VCA, inactivated antigen. |
![]() Recombinant EBV P138 Protein [His] |
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VAng-0612Lsx-inquire | Creative Biolabs | inquire | Ask for price |
Description: EBV p138 Early Antigen D (EA-D) Protein, recombinant protein from E. coli. 1.00 mg/mL. |
![]() Recombinant EBV P54 Protein [His] |
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VAng-0613Lsx-inquire | Creative Biolabs | inquire | Ask for price |
Description: EBV p54 Early Antigen D (EA-D) Protein, recombinant protein from E. coli. 1.068 mg/mL. |
![]() Recombinant EBV P18 Protein [His] |
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VAng-0614Lsx-inquire | Creative Biolabs | inquire | Ask for price |
Description: EBV Viral Capsid Antigen (VCA) p18, recombinant protein from E. coli. 2.14 mg/mL. |
![]() Recombinant EBV p23 Protein [GST] |
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VAng-0617Lsx-inquire | Creative Biolabs | inquire | Ask for price |
Description: EBV p23, recombinant protein from E. coli. 1.00 mg/mL. |
![]() EBV p18 protein (His tag) |
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80-1355S | Fitzgerald | 100 ug | EUR 241 |
Description: Purified recombinant EBV p18 protein (His tag) |
![]() EBV p54 protein (His tag) |
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80-1361 | Fitzgerald | 1 mg | EUR 1083 |
Description: Purified recombinant EBV p54 protein (His tag) |
![]() EBV p138 protein (His tag) |
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80-1362 | Fitzgerald | 1 mg | EUR 1083 |
Description: Purified recombinant EBV p138 protein (His tag) |
![]() EBV BMRF1 [GST] |
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DAG1849 | Creative Diagnostics | 500 ug | EUR 2529 |
![]() EBV Glycoprotein 125 |
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DAG3085 | Creative Diagnostics | 25 ml | EUR 1639 |
![]() Recombinant EBV p18 |
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DAGA-3074 | Creative Diagnostics | 100ug | EUR 1066 |
![]() EBV LMP2A Antibody |
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abx021712-025mg | Abbexa | 0.25 mg | EUR 1010 |
![]() Inactivated EBV Antigen |
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VAng-Lsx0109-1mL | Creative Biolabs | 1 mL | EUR 1360 |
Description: EBV, natural antigen. |
![]() EBV antibody (VCA) |
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10-E40B | Fitzgerald | 200 ug | EUR 229 |
Description: Mouse monoclonal EBV antibody (VCA) |
![]() Human EBNA1 Binding Protein 2 (EBNA1BP2) ELISA Kit |
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abx384822-96tests | Abbexa | 96 tests | EUR 911 |
![]() Mouse EBNA1 Binding Protein 2 (EBNA1BP2) ELISA Kit |
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abx389130-96tests | Abbexa | 96 tests | EUR 911 |
![]() Epstein-Barr Virus (HHV-4) Mosaic EBNA1 Protein |
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20-abx260113 | Abbexa |
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![]() EBNA1 (GFP-Puro) Lentivirus in PBS |
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LVP1134-GP-PBS | GenTarget | 1x107 IFU/ml x 200ul | EUR 710 |
Description: Concentrated lentivirus in PBS expressing Epstein Barr Virus' EBNA1 gene under EF1a promoter, containing GFP-Puromycin dual marker. |
![]() EBNA1 (RFP-Bsd) Lentivirus in PBS |
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LVP1134-RB-PBS | GenTarget | 1x107 IFU/ml x 200ul | EUR 710 |
Description: Concentrated lentivirus in PBS expressing Epstein Barr Virus' EBNA1 gene under EF1a promoter, containing RFP-Blasticidin dual marker. |