July 26, 2020

Protein Measurement Of Polyclonal Antibody

By Alexis

Measurement of protein utilizing bicinchoninic acid.

Bicinchoninic acid, sodium salt, is a steady, water-soluble compound able to forming an intense purple advanced with cuprous ion (Cu1+) in an alkaline setting. This reagent types the idea of an analytical methodology able to monitoring cuprous ion produced within the response of protein with alkaline Cu2+ (biuret response).

The colour produced from this response is steady and will increase in a proportional style over a broad vary of accelerating protein concentrations.

Recombinant Human UPK2 Protein, His, E.coli-5ug

When in comparison with the strategy of Lowry et al., the outcomes reported right here show a higher tolerance of the bicinchoninate reagent towards such generally encountered interferences as nonionic detergents and easy buffer salts. The soundness of the reagent and ensuing chromophore additionally permits for a simplified, one-step evaluation and an enhanced flexibility in protocol choice. This new methodology maintains the excessive sensitivity and low protein-to-protein variation related to the Lowry approach.

After sequence affirmation, it was transformed into E. coli BL21 (DE3) and dealt with with 0.5 mmol/L isopropyl-beta-D-thiogalactopyranoside (IPTG) to induce protein expression.
We purified and renatured the recombinant PE8 protein, and immunized New Zealand rabbits to prepare the polyclonal antibody. Antibody titer was determined by indirect ELISA and the specificity was evaluated by Western blot analysis.
Outcomes The recombinant plasmid pET28a-PE8 was effectively constructed, and the PE8 protein was primarily expressed in an inclusion physique in E. coli. After renaturation and purification, a purity of about 90% of the recombinant protein was achieved.
The titer of the polyclonal antibody was bigger than 1:430 080. The polyclonal antibody may notably acknowledge the recombinant PE8 protein.
Conclusion We’ve now effectively expressed and purified recombinant PE8 protein, which might be further utilized to generate PE8 polyclonal antibody with acceptable titer and specificity.

stat-2

UPK2 Protein Vector (Human) (pPB-N-His)

Rabies is a lethal zoonotic sickness endemic in creating worldwide areas of Asia and Africa. Simply recently, the direct speedy immunohistochemical check out (DRIT) was helpful by the World Nicely being Group (WHO) and the World Group for Animal Nicely being (OIE) as a diagnostic check out for rabies.

Because of this reality, a biotinylated polyclonal antibody (pAb) in the direction of the rabies lyssavirus (RABV) nucleoprotein was developed using a plasmid cDNA vaccine derived from an issue virus customary 11 stress. A preliminary evaluation on the efficacy of this reagent in recognizing the Philippine RABV stress was examined using banked canine hippocampal tissue samples with DRIT and the outcomes had been as compared with dFAT.

The outcomes of acetone and formalin fixation on DRIT had been moreover assessed by way of immunoreactivity scores of the specimens. Of the 142 samples examined, 104 examined optimistic and 38 harmful using every dFAT and DRIT, displaying 100% settlement between the two diagnostic procedures. Moreover, no false optimistic or false harmful outcomes had been observed using acetone and formalin fixation.

Thus, domestically prepared biotinylated pAb from plasmid cDNA may be utilized for DRIT, notably in resource-limited laboratories throughout the Philippines.

Nonetheless, these outcomes should be confirmed with a further thorough evaluation of this methodology, and the fluctuate of detection have to be further evaluated in an even bigger panel of animal samples and on completely different lyssaviruses.

Key phrases: DRIT; Evaluation; Fixation; Immunohistochemistry; Rabies; dFAT.

UPK2 Recombinant Protein (Rat)
RP235937	ABM	100 ug	Ask for price

UPK2 Uroplakin 2 Human Recombinant Protein
PROTO00526	BosterBio	Regular: 20ug	EUR 317.00
Description: UPK2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 153 amino acids (26-155 a.a) and having a molecular mass of 16.2kDa.;UPK2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Recombinant Human UPK2 Protein, His, E.coli-1mg
QP13897-1mg	EnQuireBio	1mg	EUR 2757.00

Recombinant Human UPK2 Protein, His, E.coli-20ug
QP13897-20ug	EnQuireBio	20ug	EUR 201.00

Recombinant Human UPK2 Protein, His, E.coli-5ug
QP13897-5ug	EnQuireBio	5ug	EUR 155.00

Recombinant Uroplakin 2 (UPK2)
4-RPF561Hu01	Cloud-Clone	EUR 368.80 EUR 202.00 EUR 1108.00 EUR 436.00 EUR 772.00 EUR 310.00 EUR 2620.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Uniprot ID: O00526 Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. Form: Freeze-dried powder Predicted Molecular Mass (KD): 17.4kDa Isoelectric Point: Inquire
Description: Recombinant Human Uroplakin 2 expressed in: E.coli

Human Uroplakin 2 (UPK2) Protein
20-abx650668	Abbexa	EUR 523.00 EUR 244.00 EUR 1497.00 EUR 606.00 EUR 384.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-7 working days.

UPK2 siRNA
20-abx939075	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol
Shipped within 5-10 working days.

UPK2 antibody
70R-21174	Fitzgerald	50 ul	EUR 435.00
Description: Rabbit polyclonal UPK2 antibody

UPK2 Antibody
1-CSB-PA025656GA01HU	Cusabio	EUR 597.00 EUR 333.00	150ul 50ul
Form: Liquid Buffer: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. -20â„ƒ, Avoid freeze / thaw cycles. Antigen Affinity purified
Description: A polyclonal antibody against UPK2. Recognizes UPK2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB

UPK2 protein (His tag)
30R-2967	Fitzgerald	100 ug	EUR 322.00
Description: Purified recombinant Human UPK2 protein (His tag)

Uroplakin-2 (UPK2) Protein
20-abx261408	Abbexa	EUR 3418.00 EUR 328.00 EUR 230.00	1 mg 20 ug 5 ug
Shipped within 5-10 working days.

Recombinant Human Uroplakin-2/UPK2 (C-6His)
CA11-10ug	Novoprotein	10ug	EUR 202.00
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant Human Uroplakin-2/UPK2 (C-6His)
CA11-1mg	Novoprotein	1mg	EUR 2283.00
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant Human Uroplakin-2/UPK2 (C-6His)
CA11-500ug	Novoprotein	500ug	EUR 1613.00
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant Human Uroplakin-2/UPK2 (C-6His)
CA11-50ug	Novoprotein	50ug	EUR 496.00
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

UPK2 ELISA KIT\|Human
EF004088	Lifescience Market	96 Tests	EUR 689.00

Human UPK2 shRNA Plasmid
20-abx955048	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg
Shipped within 15-20 working days.

UPK2 Polyclonal Antibody
27504-100ul	SAB	100ul	EUR 252.00

UPK2 Polyclonal Antibody
27504-50ul	SAB	50ul	EUR 187.00

UPK2 cloning plasmid
CSB-CL025656HU-10ug	Cusabio	10ug	EUR 233.00
Formulation: 10 Î¼g plasmid + 200Î¼l Glycerol Length: 555 Sequence: ATGGCACCCCTGCTGCCCATCCGGACCTTGCCCTTGATCCTGATTCTGCTGGCTCTGCTGTCCCCAGGGGCTGCAGACTTCAACATCTCAAGCCTCTCTGGTCTGCTGTCCCCGGCACTAACGGAGAGCCTGCTGGTTGCCTTGCCCCCCTGTCACCTCACAGGAGGCAATGCCAC Show more
Description: A cloning plasmid for the UPK2 gene.

anti- UPK2 antibody
FNab09274	FN Test	100µg	EUR 548.75
Recommended dilution: WB: 1:200-1:2000 IHC：1:20-1:200 Immunogen: uroplakin 2 Uniprot ID: O00526 Gene ID: 7379 Research Area: Developmental biology
Description: Antibody raised against UPK2

anti- UPK2 antibody
FNab09275	FN Test	100µg	EUR 585.00
Recommended dilution: IHC：1:20-1:200 Immunogen: uroplakin 2 Uniprot ID: O00526 Gene ID: 7379 Research Area: Developmental biology
Description: Antibody raised against UPK2

UPK2 Rabbit pAb
A10813-100ul	Abclonal	100 ul	EUR 308.00

UPK2 Rabbit pAb
A10813-200ul	Abclonal	200 ul	EUR 459.00

UPK2 Rabbit pAb
A10813-20ul	Abclonal	20 ul	EUR 183.00

UPK2 Rabbit pAb
A10813-50ul	Abclonal	50 ul	EUR 223.00

UPK2 Rabbit pAb
A3338-100ul	Abclonal	100 ul	EUR 308.00

UPK2 Rabbit pAb
A3338-200ul	Abclonal	200 ul	EUR 459.00

UPK2 Rabbit pAb
A3338-20ul	Abclonal	20 ul	Ask for price

UPK2 Rabbit pAb
A3338-50ul	Abclonal	50 ul	Ask for price

Anti-UPK2 antibody
PAab09274	Lifescience Market	100 ug	EUR 386.00

Anti-UPK2 antibody
STJ112709	St John's Laboratory	100 µl	EUR 277.00
Description: This gene encodes one of the proteins of the highly conserved urothelium-specific integral membrane proteins of the asymmetric unit membrane which forms urothelium apical plaques in mammals. The asymmetric unit membrane is believed to strengthen the urothelium by preventing cell rupture during bladder distention. The encoded protein is expressed in the peripheral blood of bladder cancer patients with transitional cell carcinomas.

Anti-UPK2 antibody
STJ26047	St John's Laboratory	100 µl	EUR 277.00
Description: This gene encodes one of the proteins of the highly conserved urothelium-specific integral membrane proteins of the asymmetric unit membrane which forms urothelium apical plaques in mammals. The asymmetric unit membrane is believed to strengthen the urothelium by preventing cell rupture during bladder distention. The encoded protein is expressed in the peripheral blood of bladder cancer patients with transitional cell carcinomas.

UPK2 ORF Vector (Human) (pORF)
ORF014895	ABM	1.0 ug DNA	EUR 354.00

UPK2 Protein Vector (Human) (pPB-C-His)
PV059577	ABM	500 ng	EUR 481.00

UPK2 Protein Vector (Human) (pPB-N-His)
PV059578	ABM	500 ng	EUR 481.00

UPK2 Protein Vector (Human) (pPM-C-HA)
PV059579	ABM	500 ng	EUR 481.00

UPK2 Protein Vector (Human) (pPM-C-His)
PV059580	ABM	500 ng	EUR 481.00

Uroplakin-2 (UPK2) Antibody
20-abx002383	Abbexa	EUR 411.00 EUR 592.00	100 ul 200 ul
Shipped within 5-10 working days.

Uroplakin 2 (UPK2) Antibody
20-abx116547	Abbexa	EUR 732.00 EUR 398.00	150 ul 50 ul
Shipped within 5-10 working days.

Uroplakin-2 (UPK2) Antibody
20-abx126762	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul
Shipped within 5-10 working days.

Uroplakin 2 (UPK2) Antibody
20-abx131673	Abbexa	EUR 453.00 EUR 133.00 EUR 1302.00 EUR 620.00 EUR 342.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-7 working days.

Uroplakin-2 (UPK2) Antibody
abx239274-100ug	Abbexa	100 ug	EUR 509.00
Shipped within 5-12 working days.

UPK2 Polyclonal Conjugated Antibody
C27504	SAB	100ul	EUR 397.00

Mouse Uroplakin-2 (Upk2)
1-CSB-EP025656MO	Cusabio	EUR 505.00 EUR 265.00 EUR 1827.00 EUR 766.00 EUR 1218.00 EUR 335.00	100ug 10ug 1MG 200ug 500ug 50ug
MW: 40.8 kDa Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Mouse Uroplakin-2(Upk2) expressed in E.coli

Human Uroplakin 2 (UPK2) ELISA Kit
20-abx156911	Abbexa	EUR 7378.00 EUR 3933.00 EUR 911.00	10 × 96 tests 5 × 96 tests 96 tests
Shipped within 5-7 working days.

Human Uroplakin 2 (UPK2) CLIA Kit
20-abx494951	Abbexa	EUR 7973.00 EUR 4246.00 EUR 981.00	10 × 96 tests 5 × 96 tests 96 tests
Please enquire.

Human Uroplakin- 2, UPK2 ELISA KIT
ELI-36740h	Lifescience Market	96 Tests	EUR 824.00

UPK2 sgRNA CRISPR Lentivector set (Human)
K2591901	ABM	3 x 1.0 ug	EUR 339.00

Uroplakin 2 (UPK2) Polyclonal Antibody (Human)
4-PAF561Hu01	Cloud-Clone	EUR 261.00 EUR 2734.00 EUR 676.00 EUR 330.00 EUR 220.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: UPK2 (Asp26~Thr153) Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Uroplakin 2 (UPK2)

Human Uroplakin 2 ELISA Kit (UPK2)
RK02481	Abclonal	96 Tests	EUR 521.00

Human Uroplakin 2(UPK2)ELISA Kit
QY-E02051	Qayee Biotechnology	96T	EUR 374.00

Human Uroplakin 2 (UPK2) ELISA Kit
SEF561Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 4731.50
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Uroplakin 2 (UPK2) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Uroplakin 2 (UPK2) in tissue homogenates, cell lysates and other biological fluids.

Human Uroplakin 2 (UPK2) ELISA Kit
SEF561Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 477.30
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Uroplakin 2 (UPK2) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Uroplakin 2 (UPK2) in tissue homogenates, cell lysates and other biological fluids.

Human Uroplakin 2 (UPK2) ELISA Kit
SEF561Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 639.00
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Uroplakin 2 (UPK2) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Uroplakin 2 (UPK2) in tissue homogenates, cell lysates and other biological fluids.

Human Uroplakin 2 (UPK2) ELISA Kit
SEF561Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2575.50
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Uroplakin 2 (UPK2) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Uroplakin 2 (UPK2) in tissue homogenates, cell lysates and other biological fluids.

Human Uroplakin 2 (UPK2) ELISA Kit
4-SEF561Hu	Cloud-Clone	EUR 4782.00 EUR 2526.00 EUR 640.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Known also as Uroplakin 2 elisa. Alternative names of the recognized antigen: UPII Uroplakin II
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Uroplakin 2 (UPK2) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

UPK2 Protein Vector (Mouse) (pPB-C-His)
PV244278	ABM	500 ng	EUR 603.00

UPK2 Protein Vector (Mouse) (pPB-N-His)
PV244279	ABM	500 ng	EUR 603.00

UPK2 Protein Vector (Mouse) (pPM-C-HA)
PV244280	ABM	500 ng	EUR 603.00

UPK2 Protein Vector (Mouse) (pPM-C-His)
PV244281	ABM	500 ng	EUR 603.00

UPK2 Protein Vector (Rat) (pPB-C-His)
PV314586	ABM	500 ng	EUR 603.00

UPK2 Protein Vector (Rat) (pPB-N-His)
PV314587	ABM	500 ng	EUR 603.00

UPK2 Protein Vector (Rat) (pPM-C-HA)
PV314588	ABM	500 ng	EUR 603.00

UPK2 Protein Vector (Rat) (pPM-C-His)
PV314589	ABM	500 ng	EUR 603.00

Uroplakin 2 (UPK2) Antibody (Cy3)
20-abx274287	Abbexa	EUR 592.00 EUR 286.00 EUR 1887.00 EUR 857.00 EUR 467.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-12 working days.

Upk2 ORF Vector (Mouse) (pORF)
ORF061070	ABM	1.0 ug DNA	EUR 506.00

Upk2 ORF Vector (Rat) (pORF)
ORF078647	ABM	1.0 ug DNA	EUR 506.00

ELISA kit for Human UPK2 (Uroplakin 2)
ELK5223	ELK Biotech	1 plate of 96 wells	EUR 432.00
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uroplakin 2 (UPK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uroplakin 2 (UP Show more
Description: A sandwich ELISA kit for detection of Uroplakin 2 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

UPK2 sgRNA CRISPR Lentivector (Human) (Target 1)
K2591902	ABM	1.0 ug DNA	EUR 154.00

UPK2 sgRNA CRISPR Lentivector (Human) (Target 2)
K2591903	ABM	1.0 ug DNA	EUR 154.00

UPK2 sgRNA CRISPR Lentivector (Human) (Target 3)
K2591904	ABM	1.0 ug DNA	EUR 154.00

Uroplakin 2 (UPK2) Polyclonal Antibody (Human), APC
4-PAF561Hu01-APC	Cloud-Clone	EUR 367.00 EUR 3581.00 EUR 989.00 EUR 470.00 EUR 228.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: UPK2 (Asp26~Thr153) Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Uroplakin 2 (UPK2). This antibody is labeled with APC.

Uroplakin 2 (UPK2) Polyclonal Antibody (Human), Biotinylated
4-PAF561Hu01-Biotin	Cloud-Clone	EUR 327.00 EUR 2684.00 EUR 783.00 EUR 403.00 EUR 225.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: UPK2 (Asp26~Thr153) Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Uroplakin 2 (UPK2). This antibody is labeled with Biotin.

Uroplakin 2 (UPK2) Polyclonal Antibody (Human), Cy3
4-PAF561Hu01-Cy3	Cloud-Clone	EUR 447.00 EUR 4733.00 EUR 1277.00 EUR 585.00 EUR 263.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: UPK2 (Asp26~Thr153) Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Uroplakin 2 (UPK2). This antibody is labeled with Cy3.

Uroplakin 2 (UPK2) Polyclonal Antibody (Human), FITC
4-PAF561Hu01-FITC	Cloud-Clone	EUR 313.00 EUR 2884.00 EUR 811.00 EUR 396.00 EUR 202.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: UPK2 (Asp26~Thr153) Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Uroplakin 2 (UPK2). This antibody is labeled with FITC.

Uroplakin 2 (UPK2) Polyclonal Antibody (Human), HRP
4-PAF561Hu01-HRP	Cloud-Clone	EUR 334.00 EUR 3120.00 EUR 873.00 EUR 424.00 EUR 214.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: UPK2 (Asp26~Thr153) Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Uroplakin 2 (UPK2). This antibody is labeled with HRP.

Uroplakin 2 (UPK2) Polyclonal Antibody (Human), PE
4-PAF561Hu01-PE	Cloud-Clone	EUR 313.00 EUR 2884.00 EUR 811.00 EUR 396.00 EUR 202.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: UPK2 (Asp26~Thr153) Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Uroplakin 2 (UPK2). This antibody is labeled with PE.

Uroplakin 2 (UPK2) Polyclonal Antibody (Human), APC-Cy7
4-PAF561Hu01-APC-Cy7	Cloud-Clone	EUR 614.00 EUR 7042.00 EUR 1858.00 EUR 821.00 EUR 337.00	100ul 10ml 1ml 200ul 20ul
Sequence of the immunogen: UPK2 (Asp26~Thr153) Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Uroplakin 2 (UPK2). This antibody is labeled with APC-Cy7.

Mouse Uroplakin- 2, Upk2 ELISA KIT
ELI-36741m	Lifescience Market	96 Tests	EUR 865.00

Porcine Uroplakin- 2, UPK2 ELISA KIT
ELI-39864p	Lifescience Market	96 Tests	EUR 928.00

Bovine Uroplakin- 2, UPK2 ELISA KIT
ELI-28839b	Lifescience Market	96 Tests	EUR 928.00

Upk2 sgRNA CRISPR Lentivector set (Rat)
K6692301	ABM	3 x 1.0 ug	EUR 339.00

Upk2 sgRNA CRISPR Lentivector set (Mouse)
K3928801	ABM	3 x 1.0 ug	EUR 339.00

AChE (acetylcholinesterase) is generally labeled as a specific biomarker of pesticide publicity. The purpose of this look at was to provide AChE polyclonal antibody from hybrid catfish that had been uncovered to industrial glyphosate.

The hybrid catfish was uncovered to glyphosate (0.75 mL/L) for 24 h. After that, the fish thoughts was dissected, AChE was extracted and purified by hydroxyapatite column chromatography and eluted with 0.2 M potassium phosphate buffer pH 6.8.

Uroplakin 2 (UPK2) (Human), APC

This protocol gave 70% yield. Then, the thoughts extract was characterised using 10% SDS-PAGE and Western blot probed with industrial polyclonal antibody explicit to AChE (PAb-AChE). The protein, 71 kDa, was then used as an antigen to immunize mice for antibody manufacturing.
The polyclonal antibody (PAb) was characterised using dot blot, Western blot and immunohistochemistry for immunolocalization of AChE in hybrid catfish uncovered to glyphosate. We found that the appropriate dilution of antibody for every dot blot and Western blot was 1:3500, and 1:2500 for immunohistochemistry.
Cross reactivity testing confirmed that PAb-AChE may be utilized with AChE from striped snakehead fish on the same dilution as used with AChE from hybrid catfish.
It was concluded that PAb explicit to hybrid catfish AChE from this work was extraordinarily explicit and delicate, and may cross-react with striped snakehead fish AChE.
Thus, this polyclonal antibody may be utilized in monitoring glyphosate publicity in hybrid catfish and striped snakehead fish.

Key phrases: Acetylcholinesterase (AChE); Biomarker; Glyphosate; Hybrid catfish; Polyclonal antibody (PAb).

Unavailability of antibodies with extreme affinity and specificity within the course of goat α_s1-casein hinders the occasion of immuno-based analytical methods equal to enzyme-linked immunosorbent assay (ELISA) and biosensors. Proper right here, we report the period of polyclonal antibodies (or immunoglobulins, IgGs) raised within the course of goat α_s1-casein N- (Nter) and C-terminal (Cter) peptide sequences.The chemical, technological and allergy properties of goat’s milk are significantly affected by the extent of α_s1-casein. Detection and quantification of α_s1-casein requires high-specificity methods to beat high-sequence similarity between this protein and others throughout the casein family.

The Nter and Cter peptides of goat α_s1-casein had been immunized in rabbits for the period of antisera, which had been purified using protein G affinity chromatography. The binding affinity of the antisera and purified IgGs had been examined and in distinction using indirect ELISA, the place peptide-BSA conjugates and goat α_s1-casein had been used as a result of the coating antigens.

ZNF287 Antibody, FITC conjugated

The Nter antiserum displayed bigger titer than Cter antiserum, at 1/64,000 and 1/32,000 dilutions, respectively. The purification step further yielded 0.5 mg/mL of purified IgGs from Three mL of antisera.

The purified Nter IgG confirmed a significantly (p < 0.05) bigger binding affinity within the course of peptide-BSA and goat α_s1-casein, with lower Okay_d price at 5.063 × 10^-3 μM as compared with 9.046 × 10^-3 μM for the Cter IgG.

A cross-reactivity check out confirmed that there was no binding in neither Nter nor Cter IgGs within the course of protein extracts from the milk of cow, buffalo, horse and camel. Extreme-quality antibodies generated will allow further development of immuno-based analytical methods and future in vitro analysis to be carried out on goat α_s1-casein.

ZNF287 Antibody
39891-100ul	SAB	100ul	EUR 390.00

ZNF287 Antibody
AF0684	Affbiotech	200ul	EUR 304.00
Description: ZNF287 Antibody detects endogenous levels of ZNF287.

ZNF287 Antibody
34071-100ul	SAB	100ul	EUR 252.00

ZNF287 Antibody
34071-50ul	SAB	50ul	EUR 187.00

ZNF287 antibody
70R-32146	Fitzgerald	100 ug	EUR 327.00
Description: Rabbit polyclonal ZNF287 antibody

ZNF287 Antibody
CSB-PA555215-	Cusabio	EUR 335.00
Form: liquid Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific Show more
Description: A polyclonal antibody against ZNF287. Recognizes ZNF287 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100

ZNF287 Antibody
CSB-PA555215-100ul	Cusabio	100ul	EUR 316.00
Form: liquid Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific Show more
Description: A polyclonal antibody against ZNF287. Recognizes ZNF287 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100

ZNF287 Antibody
1-CSB-PA040061	Cusabio	EUR 222.00 EUR 195.00	100ug 50ug
Form: Liquid Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against ZNF287. Recognizes ZNF287 from Human. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/5000

ZNF287 Antibody
1-CSB-PA026644LA01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Form: Liquid Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against ZNF287. Recognizes ZNF287 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

ZNF287 Antibody
DF3440	Affbiotech	200ul	EUR 304.00
Description: ZNF287 Antibody detects endogenous levels of total ZNF287.

ZNF287 Polyclonal Antibody
ABP55891-003ml	Abbkine	0.03ml	EUR 158.00
Immunogen information: Synthesized peptide derived from the Internal region of human ZNF287 at AA range: 210-290 Applications tips:
Description: A polyclonal antibody for detection of ZNF287 from Human. This ZNF287 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ZNF287 at AA range: 210-290

ZNF287 Polyclonal Antibody
ABP55891-01ml	Abbkine	0.1ml	EUR 289.00
Immunogen information: Synthesized peptide derived from the Internal region of human ZNF287 at AA range: 210-290 Applications tips:
Description: A polyclonal antibody for detection of ZNF287 from Human. This ZNF287 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ZNF287 at AA range: 210-290

ZNF287 Polyclonal Antibody
ABP55891-02ml	Abbkine	0.2ml	EUR 414.00
Immunogen information: Synthesized peptide derived from the Internal region of human ZNF287 at AA range: 210-290 Applications tips:
Description: A polyclonal antibody for detection of ZNF287 from Human. This ZNF287 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ZNF287 at AA range: 210-290

ZNF287 Polyclonal Antibody
A63848	EpiGentek	100 µg	EUR 570.55
Description: kits suitable for this type of research

ZNF287 Conjugated Antibody
C34071	SAB	100ul	EUR 397.00

anti- ZNF287 antibody
FNab09681	FN Test	100µg	EUR 585.00
Recommended dilution: WB: 1:200-1:1000 Immunogen: zinc finger protein 287 Uniprot ID: Q9HBT7 Gene ID: 57336 Research Area: Metabolism
Description: Antibody raised against ZNF287

ZNF287 Polyclonal Antibody
ES6890-100ul	ELK Biotech	100ul	EUR 279.00
Description: A Rabbit Polyclonal antibody against ZNF287 from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

ZNF287 Polyclonal Antibody
ES6890-50ul	ELK Biotech	50ul	EUR 207.00
Description: A Rabbit Polyclonal antibody against ZNF287 from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

ZNF287 Polyclonal Antibody
E-AB-33262-120uL	Elabscience Biotechnology	120uL	EUR 257.00
Conjugation: Unconjugated Buffer composition: PBS with 0.02% sodium azide,0.5% BSA and 50% glycerol pH 7.4. Purified by: Affinity purification Background: Zinc finger protein 287 (ZNF287) is selectively expressed by murine Th2 cells and may play a Show more
Description: Rabbit antibody against Human ZNF287 for WB,IHC-p,ELISA applications.

ZNF287 Polyclonal Antibody
E-AB-33262-20uL	Elabscience Biotechnology	20uL	EUR 130.00
Conjugation: Unconjugated Buffer composition: PBS with 0.02% sodium azide,0.5% BSA and 50% glycerol pH 7.4. Purified by: Affinity purification Background: Zinc finger protein 287 (ZNF287) is selectively expressed by murine Th2 cells and may play a Show more
Description: Rabbit antibody against Human ZNF287 for WB,IHC-p,ELISA applications.

ZNF287 Polyclonal Antibody
E-AB-33262-60uL	Elabscience Biotechnology	60uL	EUR 175.00
Conjugation: Unconjugated Buffer composition: PBS with 0.02% sodium azide,0.5% BSA and 50% glycerol pH 7.4. Purified by: Affinity purification Background: Zinc finger protein 287 (ZNF287) is selectively expressed by murine Th2 cells and may play a Show more
Description: Rabbit antibody against Human ZNF287 for WB,IHC-p,ELISA applications.

Anti-ZNF287 antibody
PAab09681	Lifescience Market	100 ug	EUR 412.00

Anti-ZNF287 antibody
STJ96325	St John's Laboratory	200 µl	EUR 197.00
Description: Rabbit polyclonal to ZNF287.

ZNF287 siRNA
20-abx940672	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol
Shipped within 5-10 working days.

ZNF287 siRNA
20-abx940673	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol
Shipped within 5-10 working days.

Anti-ZNF287/Zfp287 Antibody
A17720	BosterBio	100ul	EUR 397.00
Description: Rabbit Polyclonal ZNF287/Zfp287 Antibody. Validated in IHC, WB and tested in Human, Mouse.

ZNF287 Antibody, HRP conjugated
1-CSB-PA026644LB01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Form: Liquid Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against ZNF287. Recognizes ZNF287 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

ZNF287 Antibody, FITC conjugated
1-CSB-PA026644LC01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Form: Liquid Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against ZNF287. Recognizes ZNF287 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

ZNF287 Antibody, Biotin conjugated
1-CSB-PA026644LD01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Form: Liquid Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against ZNF287. Recognizes ZNF287 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

ZNF287 ELISA KIT\|Human
EF004450	Lifescience Market	96 Tests	EUR 689.00

Human ZNF287 shRNA Plasmid
20-abx961417	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg
Shipped within 15-20 working days.

ZNF287 Recombinant Protein (Human)
RP045112	ABM	100 ug	Ask for price

ZNF287 Blocking Peptide
AF0684-BP	Affbiotech	1mg	EUR 195.00

ZNF287 cloning plasmid
CSB-CL026644HU-10ug	Cusabio	10ug	EUR 474.00
Formulation: 10 Î¼g plasmid + 200Î¼l Glycerol Length: 2265 Sequence: ATGAACAGTTCTTCACGTTCTCAAATCCTTCTAAGGTGGAAGTCAGACAAGGCTCAGAGTGGACCCTACAATGTTGAGAAGGAAATCCTTACTTCAAGATTCTTGCGTGACACTGAGACCTGTCGACAGAATTTTAGGAATTTTCCATACCCAGACCTGGCTGGTCCTCGAAAGG Show more
Description: A cloning plasmid for the ZNF287 gene.

ZNF287 Blocking Peptide
DF3440-BP	Affbiotech	1mg	EUR 195.00

ZNF287 Polyclonal Antibody, HRP Conjugated
A63849	EpiGentek	100 µg	EUR 570.55
Description: fast delivery possible

ZNF287 Polyclonal Antibody, FITC Conjugated
A63850	EpiGentek	100 µg	EUR 570.55
Description: reagents widely cited

ZNF287 Polyclonal Antibody, Biotin Conjugated
A63851	EpiGentek	100 µg	EUR 570.55
Description: Ask the seller for details

ZNF287 ORF Vector (Human) (pORF)
ORF015038	ABM	1.0 ug DNA	EUR 354.00

Mouse ZNF287 shRNA Plasmid
20-abx980321	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg
Shipped within 15-20 working days.

Zinc Finger Protein 287 (ZNF287) Antibody
abx030175-400ul	Abbexa	400 ul	EUR 523.00
Shipped within 5-10 working days.

Zinc Finger Protein 287 (ZNF287) Antibody
abx030175-80l	Abbexa	80 µl	EUR 286.00
Shipped within 5-10 working days.

Zinc Finger Protein 287 (ZNF287) Antibody
abx037179-100ug	Abbexa	100 ug	EUR 391.00
Shipped within 5-10 working days.

Zinc Finger Protein 287 (ZNF287) Antibody
20-abx013803	Abbexa	EUR 314.00 EUR 98.00 EUR 398.00 EUR 495.00	100 ug 10 ug 200 ug 300 µg
Shipped within 5-10 working days.

Zinc Finger Protein 287 (ZNF287) Antibody
20-abx306051	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug
Shipped within 5-10 working days.

Zinc Finger Protein 287 (ZNF287) Antibody
abx330676-100ul	Abbexa	100 ul	EUR 425.00
Shipped within 5-10 working days.

Zinc Finger Protein 287 (ZNF287) Antibody
20-abx324762	Abbexa	EUR 314.00 EUR 244.00	100 ug 50 ug
Shipped within 5-10 working days.

Zinc Finger Protein 287 (ZNF287) Antibody
abx239681-100ug	Abbexa	100 ug	EUR 551.00
Shipped within 5-12 working days.

ZNF287 sgRNA CRISPR Lentivector set (Human)
K2697601	ABM	3 x 1.0 ug	EUR 339.00

Zinc Finger Protein 287 (ZNF287) Antibody (HRP)
20-abx306052	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug
Shipped within 5-10 working days.

Zinc Finger Protein 287 (ZNF287) Antibody (FITC)
20-abx306053	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug
Shipped within 5-10 working days.

Zinc Finger Protein 287 (ZNF287) Antibody (Biotin)
20-abx306054	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug
Shipped within 5-10 working days.

ZNF287 sgRNA CRISPR Lentivector (Human) (Target 1)
K2697602	ABM	1.0 ug DNA	EUR 154.00

ZNF287 sgRNA CRISPR Lentivector (Human) (Target 2)
K2697603	ABM	1.0 ug DNA	EUR 154.00

ZNF287 sgRNA CRISPR Lentivector (Human) (Target 3)
K2697604	ABM	1.0 ug DNA	EUR 154.00

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Protein Measurement Of Polyclonal Antibody

Measurement of protein utilizing bicinchoninic acid.

Recombinant Human UPK2 Protein, His, E.coli-5ug

UPK2 Protein Vector (Human) (pPB-N-His)

UPK2 Recombinant Protein (Rat)

UPK2 Uroplakin 2 Human Recombinant Protein

Recombinant Human UPK2 Protein, His, E.coli-1mg

Recombinant Human UPK2 Protein, His, E.coli-20ug

Recombinant Human UPK2 Protein, His, E.coli-5ug

Recombinant Uroplakin 2 (UPK2)

Human Uroplakin 2 (UPK2) Protein

UPK2 siRNA

UPK2 antibody

UPK2 Antibody

UPK2 protein (His tag)

Uroplakin-2 (UPK2) Protein

Recombinant Human Uroplakin-2/UPK2 (C-6His)

Recombinant Human Uroplakin-2/UPK2 (C-6His)

Recombinant Human Uroplakin-2/UPK2 (C-6His)

Recombinant Human Uroplakin-2/UPK2 (C-6His)

UPK2 ELISA KIT|Human

Human UPK2 shRNA Plasmid

UPK2 Polyclonal Antibody

UPK2 Polyclonal Antibody

UPK2 cloning plasmid

anti- UPK2 antibody

anti- UPK2 antibody

UPK2 Rabbit pAb

UPK2 Rabbit pAb

UPK2 Rabbit pAb

UPK2 Rabbit pAb

UPK2 Rabbit pAb

UPK2 Rabbit pAb

UPK2 Rabbit pAb

UPK2 Rabbit pAb

Anti-UPK2 antibody

Anti-UPK2 antibody

Anti-UPK2 antibody

UPK2 ORF Vector (Human) (pORF)

UPK2 Protein Vector (Human) (pPB-C-His)

UPK2 Protein Vector (Human) (pPB-N-His)

UPK2 Protein Vector (Human) (pPM-C-HA)

UPK2 Protein Vector (Human) (pPM-C-His)

Uroplakin-2 (UPK2) Antibody

Uroplakin 2 (UPK2) Antibody

Uroplakin-2 (UPK2) Antibody

Uroplakin 2 (UPK2) Antibody

Uroplakin-2 (UPK2) Antibody

UPK2 Polyclonal Conjugated Antibody

Mouse Uroplakin-2 (Upk2)

Human Uroplakin 2 (UPK2) ELISA Kit

Human Uroplakin 2 (UPK2) CLIA Kit

Human Uroplakin- 2, UPK2 ELISA KIT

UPK2 sgRNA CRISPR Lentivector set (Human)

Uroplakin 2 (UPK2) Polyclonal Antibody (Human)

Human Uroplakin 2 ELISA Kit (UPK2)

Human Uroplakin 2(UPK2)ELISA Kit

Human Uroplakin 2 (UPK2) ELISA Kit

Human Uroplakin 2 (UPK2) ELISA Kit

Human Uroplakin 2 (UPK2) ELISA Kit

Human Uroplakin 2 (UPK2) ELISA Kit

Human Uroplakin 2 (UPK2) ELISA Kit

UPK2 Protein Vector (Mouse) (pPB-C-His)

UPK2 Protein Vector (Mouse) (pPB-N-His)

UPK2 Protein Vector (Mouse) (pPM-C-HA)

UPK2 Protein Vector (Mouse) (pPM-C-His)

UPK2 Protein Vector (Rat) (pPB-C-His)

UPK2 Protein Vector (Rat) (pPB-N-His)

UPK2 Protein Vector (Rat) (pPM-C-HA)

UPK2 Protein Vector (Rat) (pPM-C-His)

Uroplakin 2 (UPK2) Antibody (Cy3)

Upk2 ORF Vector (Mouse) (pORF)

Upk2 ORF Vector (Rat) (pORF)

ELISA kit for Human UPK2 (Uroplakin 2)

UPK2 sgRNA CRISPR Lentivector (Human) (Target 1)

UPK2 sgRNA CRISPR Lentivector (Human) (Target 2)

UPK2 sgRNA CRISPR Lentivector (Human) (Target 3)

Uroplakin 2 (UPK2) Polyclonal Antibody (Human), APC

Uroplakin 2 (UPK2) Polyclonal Antibody (Human), Biotinylated

Uroplakin 2 (UPK2) Polyclonal Antibody (Human), Cy3