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July 26, 2020

Protein Measurement Of Polyclonal Antibody

By Alexis

Measurement of protein utilizing bicinchoninic acid.

Bicinchoninic acid, sodium salt, is a steady, water-soluble compound able to forming an intense purple advanced with cuprous ion (Cu1+) in an alkaline setting. This reagent types the idea of an analytical methodology able to monitoring cuprous ion produced within the response of protein with alkaline Cu2+ (biuret response).
The colour produced from this response is steady and will increase in a proportional style over a broad vary of accelerating protein concentrations.

Recombinant Human UPK2 Protein, His, E.coli-5ug
When in comparison with the strategy of Lowry et al., the outcomes reported right here show a higher tolerance of the bicinchoninate reagent towards such generally encountered interferences as nonionic detergents and easy buffer salts. The soundness of the reagent and ensuing chromophore additionally permits for a simplified, one-step evaluation and an enhanced flexibility in protocol choice. This new methodology maintains the excessive sensitivity and low protein-to-protein variation related to the Lowry approach.

 

  • After sequence affirmation, it was transformed into E. coli BL21 (DE3) and dealt with with 0.5 mmol/L isopropyl-beta-D-thiogalactopyranoside (IPTG) to induce protein expression.
  • We purified and renatured the recombinant PE8 protein, and immunized New Zealand rabbits to prepare the polyclonal antibody. Antibody titer was determined by indirect ELISA and the specificity was evaluated by Western blot analysis.
  • Outcomes The recombinant plasmid pET28a-PE8 was effectively constructed, and the PE8 protein was primarily expressed in an inclusion physique in E. coli. After renaturation and purification, a purity of about 90% of the recombinant protein was achieved.
  • The titer of the polyclonal antibody was bigger than 1:430 080. The polyclonal antibody may notably acknowledge the recombinant PE8 protein.
  • Conclusion We’ve now effectively expressed and purified recombinant PE8 protein, which might be further utilized to generate PE8 polyclonal antibody with acceptable titer and specificity.
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UPK2 Protein Vector (Human) (pPB-N-His)

 

 

Rabies is a lethal zoonotic sickness endemic in creating worldwide areas of Asia and Africa. Simply recently, the direct speedy immunohistochemical check out (DRIT) was helpful by the World Nicely being Group (WHO) and the World Group for Animal Nicely being (OIE) as a diagnostic check out for rabies.

Because of this reality, a biotinylated polyclonal antibody (pAb) in the direction of the rabies lyssavirus (RABV) nucleoprotein was developed using a plasmid cDNA vaccine derived from an issue virus customary 11 stress. A preliminary evaluation on the efficacy of this reagent in recognizing the Philippine RABV stress was examined using banked canine hippocampal tissue samples with DRIT and the outcomes had been as compared with dFAT.

The outcomes of acetone and formalin fixation on DRIT had been moreover assessed by way of immunoreactivity scores of the specimens. Of the 142 samples examined, 104 examined optimistic and 38 harmful using every dFAT and DRIT, displaying 100% settlement between the two diagnostic procedures. Moreover, no false optimistic or false harmful outcomes had been observed using acetone and formalin fixation.

Thus, domestically prepared biotinylated pAb from plasmid cDNA may be utilized for DRIT, notably in resource-limited laboratories throughout the Philippines.

Nonetheless, these outcomes should be confirmed with a further thorough evaluation of this methodology, and the fluctuate of detection have to be further evaluated in an even bigger panel of animal samples and on completely different lyssaviruses.

Key phrases: DRIT; Evaluation; Fixation; Immunohistochemistry; Rabies; dFAT.

 

UPK2 Recombinant Protein (Mouse)
RP183206 100 ug Ask for price

UPK2 Uroplakin 2 Human Recombinant Protein
PROTO00526 Regular: 20ug
EUR 317
Description: UPK2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 153 amino acids (26-155 a.a) and having a molecular mass of 16.2kDa.;UPK2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Recombinant Human UPK2 Protein, His, E.coli-1mg
QP13897-1mg 1mg
EUR 2757

Recombinant Human UPK2 Protein, His, E.coli-20ug
QP13897-20ug 20ug
EUR 201

Recombinant Human UPK2 Protein, His, E.coli-5ug
QP13897-5ug 5ug
EUR 155

Recombinant Uroplakin 2 (UPK2)
4-RPF561Hu01
  • EUR 368.80
  • EUR 202.00
  • EUR 1108.00
  • EUR 436.00
  • EUR 772.00
  • EUR 310.00
  • EUR 2620.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: O00526
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 17.4kDa
  • Isoelectric Point: Inquire
Description: Recombinant Human Uroplakin 2 expressed in: E.coli

Human Uroplakin 2 (UPK2) Protein
20-abx650668
  • EUR 523.00
  • EUR 244.00
  • EUR 1497.00
  • EUR 606.00
  • EUR 384.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

UPK2 siRNA
20-abx939075
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

UPK2 antibody
70R-21174 50 ul
EUR 435
Description: Rabbit polyclonal UPK2 antibody

UPK2 Antibody
1-CSB-PA025656GA01HU
  • EUR 597.00
  • EUR 333.00
  • 150ul
  • 50ul
  • Form: Liquid
  • Buffer: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. -20℃, Avoid freeze / thaw cycles. Antigen Affinity purified
Description: A polyclonal antibody against UPK2. Recognizes UPK2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB

Uroplakin-2 (UPK2) Protein
20-abx261408
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

UPK2 protein (His tag)
30R-2967 100 ug
EUR 322
Description: Purified recombinant Human UPK2 protein (His tag)

Recombinant Human Uroplakin-2/UPK2 (C-6His)
CA11-10ug 10ug
EUR 202
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant Human Uroplakin-2/UPK2 (C-6His)
CA11-1mg 1mg
EUR 2283
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant Human Uroplakin-2/UPK2 (C-6His)
CA11-500ug 500ug
EUR 1613
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant Human Uroplakin-2/UPK2 (C-6His)
CA11-50ug 50ug
EUR 496
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

UPK2 ELISA KIT|Human
EF004088 96 Tests
EUR 689

Human UPK2 shRNA Plasmid
20-abx955048
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

UPK2 cloning plasmid
CSB-CL025656HU-10ug 10ug
EUR 233
  • Formulation: 10 μg plasmid + 200μl Glycerol
  • Length: 555
  • Sequence: ATGGCACCCCTGCTGCCCATCCGGACCTTGCCCTTGATCCTGATTCTGCTGGCTCTGCTGTCCCCAGGGGCTGCAGACTTCAACATCTCAAGCCTCTCTGGTCTGCTGTCCCCGGCACTAACGGAGAGCCTGCTGGTTGCCTTGCCCCCCTGTCACCTCACAGGAGGCAATGCCAC
  • Show more
Description: A cloning plasmid for the UPK2 gene.

anti- UPK2 antibody
FNab09274 100µg
EUR 548.75
  • Recommended dilution: WB: 1:200-1:2000
  • IHC：1:20-1:200
  • Immunogen: uroplakin 2
  • Uniprot ID: O00526
  • Gene ID: 7379
  • Research Area: Developmental biology
Description: Antibody raised against UPK2

anti- UPK2 antibody
FNab09275 100µg
EUR 585
  • Recommended dilution: IHC：1:20-1:200
  • Immunogen: uroplakin 2
  • Uniprot ID: O00526
  • Gene ID: 7379
  • Research Area: Developmental biology
Description: Antibody raised against UPK2

UPK2 Rabbit pAb
A10813-100ul 100 ul
EUR 308

UPK2 Rabbit pAb
A10813-200ul 200 ul
EUR 459

UPK2 Rabbit pAb
A10813-20ul 20 ul
EUR 183

UPK2 Rabbit pAb
A10813-50ul 50 ul
EUR 223

UPK2 Rabbit pAb
A3338-100ul 100 ul
EUR 308

UPK2 Rabbit pAb
A3338-200ul 200 ul
EUR 459

UPK2 Rabbit pAb
A3338-20ul 20 ul Ask for price

UPK2 Rabbit pAb
A3338-50ul 50 ul Ask for price

UPK2 Polyclonal Antibody
27504-100ul 100ul
EUR 252

UPK2 Polyclonal Antibody
27504-50ul 50ul
EUR 187

Anti-UPK2 antibody
PAab09274 100 ug
EUR 386

Anti-UPK2 antibody
STJ26047 100 µl
EUR 277
Description: This gene encodes one of the proteins of the highly conserved urothelium-specific integral membrane proteins of the asymmetric unit membrane which forms urothelium apical plaques in mammals. The asymmetric unit membrane is believed to strengthen the urothelium by preventing cell rupture during bladder distention. The encoded protein is expressed in the peripheral blood of bladder cancer patients with transitional cell carcinomas.

Anti-UPK2 antibody
STJ112709 100 µl
EUR 277
Description: This gene encodes one of the proteins of the highly conserved urothelium-specific integral membrane proteins of the asymmetric unit membrane which forms urothelium apical plaques in mammals. The asymmetric unit membrane is believed to strengthen the urothelium by preventing cell rupture during bladder distention. The encoded protein is expressed in the peripheral blood of bladder cancer patients with transitional cell carcinomas.

UPK2 ORF Vector (Human) (pORF)
ORF014895 1.0 ug DNA
EUR 354

UPK2 ELISA Kit (Human) (OKCD00470)
OKCD00470 96 Wells
EUR 831
Description: Description of target: Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in regulating the assembly of the AUM (By similarity).By similarity ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.053 ng/mL

UPK2 ELISA Kit (Human) (OKAN06386)
OKAN06386 96 Wells
EUR 792
Description: Description of target: This gene encodes one of the proteins of the highly conserved urothelium-specific integral membrane proteins of the asymmetric unit membrane which forms urothelium apical plaques in mammals. The asymmetric unit membrane is believed to strengthen the urothelium by preventing cell rupture during bladder distention. The encoded protein is expressed in the peripheral blood of bladder cancer patients with transitional cell carcinomas.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.053 ng/mL

UPK2 Protein Vector (Human) (pPB-C-His)
PV059577 500 ng
EUR 481

UPK2 Protein Vector (Human) (pPB-N-His)
PV059578 500 ng
EUR 481

UPK2 Protein Vector (Human) (pPM-C-HA)
PV059579 500 ng
EUR 481

UPK2 Protein Vector (Human) (pPM-C-His)
PV059580 500 ng
EUR 481

UPK2 Polyclonal Conjugated Antibody
C27504 100ul
EUR 397

Uroplakin-2 (UPK2) Antibody
20-abx126762
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

Uroplakin 2 (UPK2) Antibody
20-abx116547
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.

Uroplakin 2 (UPK2) Antibody
20-abx131673
  • EUR 453.00
  • EUR 133.00
  • EUR 1302.00
  • EUR 620.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Uroplakin-2 (UPK2) Antibody
20-abx002383
  • EUR 411.00
  • EUR 592.00
  • 100 ul
  • 200 ul
  • Shipped within 5-10 working days.

Uroplakin-2 (UPK2) Antibody
abx239274-100ug 100 ug
EUR 509
  • Shipped within 5-12 working days.

Mouse Uroplakin-2 (Upk2)
1-CSB-EP025656MO
  • EUR 505.00
  • EUR 265.00
  • EUR 1827.00
  • EUR 766.00
  • EUR 1218.00
  • EUR 335.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 40.8 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Mouse Uroplakin-2(Upk2) expressed in E.coli

Human Uroplakin- 2, UPK2 ELISA KIT
ELI-36740h 96 Tests
EUR 824

Human Uroplakin 2 (UPK2) ELISA Kit
20-abx156911
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.

Human Uroplakin 2 (UPK2) CLIA Kit
20-abx494951
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Please enquire.

UPK2 sgRNA CRISPR Lentivector set (Human)
K2591901 3 x 1.0 ug
EUR 339

Uroplakin 2 (UPK2) Polyclonal Antibody (Human)
4-PAF561Hu01
  • EUR 261.00
  • EUR 2734.00
  • EUR 676.00
  • EUR 330.00
  • EUR 220.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: UPK2 (Asp26~Thr153)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Uroplakin 2 (UPK2)

Human Uroplakin 2 ELISA Kit (UPK2)
RK02481 96 Tests
EUR 521

Human Uroplakin 2(UPK2)ELISA Kit
QY-E02051 96T
EUR 374

Human Uroplakin 2 (UPK2) ELISA Kit
SEF561Hu-10x96wellstestplate 10x96-wells test plate
EUR 4731.5
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Uroplakin 2 (UPK2) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Uroplakin 2 (UPK2) in tissue homogenates, cell lysates and other biological fluids.

Human Uroplakin 2 (UPK2) ELISA Kit
SEF561Hu-1x48wellstestplate 1x48-wells test plate
EUR 477.3
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Uroplakin 2 (UPK2) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Uroplakin 2 (UPK2) in tissue homogenates, cell lysates and other biological fluids.

Human Uroplakin 2 (UPK2) ELISA Kit
SEF561Hu-1x96wellstestplate 1x96-wells test plate
EUR 639
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Uroplakin 2 (UPK2) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Uroplakin 2 (UPK2) in tissue homogenates, cell lysates and other biological fluids.

Human Uroplakin 2 (UPK2) ELISA Kit
SEF561Hu-5x96wellstestplate 5x96-wells test plate
EUR 2575.5
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Uroplakin 2 (UPK2) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Uroplakin 2 (UPK2) in tissue homogenates, cell lysates and other biological fluids.

Human Uroplakin 2 (UPK2) ELISA Kit
4-SEF561Hu
  • EUR 4782.00
  • EUR 2526.00
  • EUR 640.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
  • Known also as Uroplakin 2 elisa. Alternative names of the recognized antigen: UPII
  • Uroplakin II
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Uroplakin 2 (UPK2) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

UPK2 Protein Vector (Rat) (pPB-C-His)
PV314586 500 ng
EUR 603

UPK2 Protein Vector (Rat) (pPB-N-His)
PV314587 500 ng
EUR 603

UPK2 Protein Vector (Rat) (pPM-C-HA)
PV314588 500 ng
EUR 603

UPK2 Protein Vector (Rat) (pPM-C-His)
PV314589 500 ng
EUR 603

UPK2 Protein Vector (Mouse) (pPB-C-His)
PV244278 500 ng
EUR 603

UPK2 Protein Vector (Mouse) (pPB-N-His)
PV244279 500 ng
EUR 603

UPK2 Protein Vector (Mouse) (pPM-C-HA)
PV244280 500 ng
EUR 603

UPK2 Protein Vector (Mouse) (pPM-C-His)
PV244281 500 ng
EUR 603

Uroplakin 2 (UPK2) Antibody (Cy3)
20-abx274287
  • EUR 592.00
  • EUR 286.00
  • EUR 1887.00
  • EUR 857.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-12 working days.

Upk2 ORF Vector (Rat) (pORF)
ORF078647 1.0 ug DNA
EUR 506

Upk2 ORF Vector (Mouse) (pORF)
ORF061070 1.0 ug DNA
EUR 506

ELISA kit for Human UPK2 (Uroplakin 2)
ELK5223 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uroplakin 2 (UPK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uroplakin 2 (UP
  • Show more
Description: A sandwich ELISA kit for detection of Uroplakin 2 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

UPK2 sgRNA CRISPR Lentivector (Human) (Target 1)
K2591902 1.0 ug DNA
EUR 154

UPK2 sgRNA CRISPR Lentivector (Human) (Target 2)
K2591903 1.0 ug DNA
EUR 154

UPK2 sgRNA CRISPR Lentivector (Human) (Target 3)
K2591904 1.0 ug DNA
EUR 154

Uroplakin 2 (UPK2) Polyclonal Antibody (Human), APC
4-PAF561Hu01-APC
  • EUR 367.00
  • EUR 3581.00
  • EUR 989.00
  • EUR 470.00
  • EUR 228.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: UPK2 (Asp26~Thr153)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Uroplakin 2 (UPK2). This antibody is labeled with APC.

Uroplakin 2 (UPK2) Polyclonal Antibody (Human), Biotinylated
4-PAF561Hu01-Biotin
  • EUR 327.00
  • EUR 2684.00
  • EUR 783.00
  • EUR 403.00
  • EUR 225.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: UPK2 (Asp26~Thr153)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Uroplakin 2 (UPK2). This antibody is labeled with Biotin.

Uroplakin 2 (UPK2) Polyclonal Antibody (Human), Cy3
4-PAF561Hu01-Cy3
  • EUR 447.00
  • EUR 4733.00
  • EUR 1277.00
  • EUR 585.00
  • EUR 263.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: UPK2 (Asp26~Thr153)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Uroplakin 2 (UPK2). This antibody is labeled with Cy3.

Uroplakin 2 (UPK2) Polyclonal Antibody (Human), FITC
4-PAF561Hu01-FITC
  • EUR 313.00
  • EUR 2884.00
  • EUR 811.00
  • EUR 396.00
  • EUR 202.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: UPK2 (Asp26~Thr153)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Uroplakin 2 (UPK2). This antibody is labeled with FITC.

Uroplakin 2 (UPK2) Polyclonal Antibody (Human), HRP
4-PAF561Hu01-HRP
  • EUR 334.00
  • EUR 3120.00
  • EUR 873.00
  • EUR 424.00
  • EUR 214.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: UPK2 (Asp26~Thr153)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Uroplakin 2 (UPK2). This antibody is labeled with HRP.

Uroplakin 2 (UPK2) Polyclonal Antibody (Human), PE
4-PAF561Hu01-PE
  • EUR 313.00
  • EUR 2884.00
  • EUR 811.00
  • EUR 396.00
  • EUR 202.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: UPK2 (Asp26~Thr153)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Uroplakin 2 (UPK2). This antibody is labeled with PE.

Uroplakin 2 (UPK2) Polyclonal Antibody (Human), APC-Cy7
4-PAF561Hu01-APC-Cy7
  • EUR 614.00
  • EUR 7042.00
  • EUR 1858.00
  • EUR 821.00
  • EUR 337.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: UPK2 (Asp26~Thr153)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Uroplakin 2 (UPK2). This antibody is labeled with APC-Cy7.

Mouse Uroplakin- 2, Upk2 ELISA KIT
ELI-36741m 96 Tests
EUR 865

Bovine Uroplakin- 2, UPK2 ELISA KIT
ELI-28839b 96 Tests
EUR 928

Porcine Uroplakin- 2, UPK2 ELISA KIT
ELI-39864p 96 Tests
EUR 928

AChE (acetylcholinesterase) is generally labeled as a specific biomarker of pesticide publicity. The purpose of this look at was to provide AChE polyclonal antibody from hybrid catfish that had been uncovered to industrial glyphosate.

The hybrid catfish was uncovered to glyphosate (0.75 mL/L) for 24 h. After that, the fish thoughts was dissected, AChE was extracted and purified by hydroxyapatite column chromatography and eluted with 0.2 M potassium phosphate buffer pH 6.8.

Uroplakin 2 (UPK2)  (Human), APC

  • This protocol gave 70% yield. Then, the thoughts extract was characterised using 10% SDS-PAGE and Western blot probed with industrial polyclonal antibody explicit to AChE (PAb-AChE). The protein, 71 kDa, was then used as an antigen to immunize mice for antibody manufacturing.
  • The polyclonal antibody (PAb) was characterised using dot blot, Western blot and immunohistochemistry for immunolocalization of AChE in hybrid catfish uncovered to glyphosate. We found that the appropriate dilution of antibody for every dot blot and Western blot was 1:3500, and 1:2500 for immunohistochemistry.
  • Cross reactivity testing confirmed that PAb-AChE may be utilized with AChE from striped snakehead fish on the same dilution as used with AChE from hybrid catfish.
  • It was concluded that PAb explicit to hybrid catfish AChE from this work was extraordinarily explicit and delicate, and may cross-react with striped snakehead fish AChE.
  • Thus, this polyclonal antibody may be utilized in monitoring glyphosate publicity in hybrid catfish and striped snakehead fish.
Key phrases: Acetylcholinesterase (AChE); Biomarker; Glyphosate; Hybrid catfish; Polyclonal antibody (PAb).

 

Unavailability of antibodies with extreme affinity and specificity within the course of goat αs1-casein hinders the occasion of immuno-based analytical methods equal to enzyme-linked immunosorbent assay (ELISA) and biosensors. Proper right here, we report the period of polyclonal antibodies (or immunoglobulins, IgGs) raised within the course of goat αs1-casein N- (Nter) and C-terminal (Cter) peptide sequences.The chemical, technological and allergy properties of goat’s milk are significantly affected by the extent of αs1-casein. Detection and quantification of αs1-casein requires high-specificity methods to beat high-sequence similarity between this protein and others throughout the casein family.

The Nter and Cter peptides of goat αs1-casein had been immunized in rabbits for the period of antisera, which had been purified using protein G affinity chromatography. The binding affinity of the antisera and purified IgGs had been examined and in distinction using indirect ELISA, the place peptide-BSA conjugates and goat αs1-casein had been used as a result of the coating antigens.

ZNF287 Antibody, FITC conjugated

 

The Nter antiserum displayed bigger titer than Cter antiserum, at 1/64,000 and 1/32,000 dilutions, respectively. The purification step further yielded 0.5 mg/mL of purified IgGs from Three mL of antisera.

The purified Nter IgG confirmed a significantly (p < 0.05) bigger binding affinity within the course of peptide-BSA and goat αs1-casein, with lower Okayd price at 5.063 × 10-3 μM as compared with 9.046 × 10-3 μM for the Cter IgG.

A cross-reactivity check out confirmed that there was no binding in neither Nter nor Cter IgGs within the course of protein extracts from the milk of cow, buffalo, horse and camel. Extreme-quality antibodies generated will allow further development of immuno-based analytical methods and future in vitro analysis to be carried out on goat αs1-casein.

 

ZNF287 antibody
70R-32146 100 ug
EUR 327
Description: Rabbit polyclonal ZNF287 antibody

ZNF287 Antibody
ABD3440 100 ug
EUR 438

ZNF287 Antibody
39891-100ul 100ul
EUR 390

ZNF287 Antibody
34071-100ul 100ul
EUR 252

ZNF287 Antibody
34071-50ul 50ul
EUR 187

ZNF287 Antibody
DF3440 200ul
EUR 304
Description: ZNF287 Antibody detects endogenous levels of total ZNF287.

ZNF287 Antibody
CSB-PA555215-
EUR 335
  • Form: liquid
  • Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific
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Description: A polyclonal antibody against ZNF287. Recognizes ZNF287 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100

ZNF287 Antibody
CSB-PA555215-100ul 100ul
EUR 316
  • Form: liquid
  • Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific
  • Show more
Description: A polyclonal antibody against ZNF287. Recognizes ZNF287 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100

ZNF287 Antibody
1-CSB-PA040061
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against ZNF287. Recognizes ZNF287 from Human. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/5000

ZNF287 Antibody
1-CSB-PA026644LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against ZNF287. Recognizes ZNF287 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

ZNF287 Conjugated Antibody
C34071 100ul
EUR 397

anti- ZNF287 antibody
FNab09681 100µg
EUR 585
  • Recommended dilution: WB: 1:200-1:1000
  • Immunogen: zinc finger protein 287
  • Uniprot ID: Q9HBT7
  • Gene ID: 57336
  • Research Area: Metabolism
Description: Antibody raised against ZNF287

ZNF287 Polyclonal Antibody
ES6890-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against ZNF287 from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

ZNF287 Polyclonal Antibody
ES6890-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against ZNF287 from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

ZNF287 Polyclonal Antibody
ABP55891-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from the Internal region of human ZNF287 at AA range: 210-290
  • Applications tips:
Description: A polyclonal antibody for detection of ZNF287 from Human. This ZNF287 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ZNF287 at AA range: 210-290

ZNF287 Polyclonal Antibody
ABP55891-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from the Internal region of human ZNF287 at AA range: 210-290
  • Applications tips:
Description: A polyclonal antibody for detection of ZNF287 from Human. This ZNF287 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ZNF287 at AA range: 210-290

ZNF287 Polyclonal Antibody
ABP55891-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from the Internal region of human ZNF287 at AA range: 210-290
  • Applications tips:
Description: A polyclonal antibody for detection of ZNF287 from Human. This ZNF287 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human ZNF287 at AA range: 210-290

ZNF287 Polyclonal Antibody
A63848 100 µg
EUR 570.55
Description: kits suitable for this type of research

Anti-ZNF287 antibody
PAab09681 100 ug
EUR 412

Anti-ZNF287 antibody
STJ96325 200 µl
EUR 197
Description: Rabbit polyclonal to ZNF287.

ZNF287 siRNA
20-abx940672
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

ZNF287 siRNA
20-abx940673
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

Anti-ZNF287/Zfp287 Antibody
A17720 100ul
EUR 397
Description: Rabbit Polyclonal ZNF287/Zfp287 Antibody. Validated in IHC, WB and tested in Human, Mouse.

ZNF287 Antibody, HRP conjugated
1-CSB-PA026644LB01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against ZNF287. Recognizes ZNF287 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

ZNF287 Antibody, FITC conjugated
1-CSB-PA026644LC01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against ZNF287. Recognizes ZNF287 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

ZNF287 Antibody, Biotin conjugated
1-CSB-PA026644LD01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against ZNF287. Recognizes ZNF287 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

ZNF287 ELISA KIT|Human
EF004450 96 Tests
EUR 689

Human ZNF287 shRNA Plasmid
20-abx961417
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

ZNF287 Recombinant Protein (Human)
RP045112 100 ug Ask for price

ZNF287 Blocking Peptide
AF0684-BP 1mg
EUR 195

ZNF287 cloning plasmid
CSB-CL026644HU-10ug 10ug
EUR 474
  • Formulation: 10 μg plasmid + 200μl Glycerol
  • Length: 2265
  • Sequence: ATGAACAGTTCTTCACGTTCTCAAATCCTTCTAAGGTGGAAGTCAGACAAGGCTCAGAGTGGACCCTACAATGTTGAGAAGGAAATCCTTACTTCAAGATTCTTGCGTGACACTGAGACCTGTCGACAGAATTTTAGGAATTTTCCATACCCAGACCTGGCTGGTCCTCGAAAGG
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Description: A cloning plasmid for the ZNF287 gene.

ZNF287 Blocking Peptide
DF3440-BP 1mg
EUR 195

ZNF287 Polyclonal Antibody, HRP Conjugated
A63849 100 µg
EUR 570.55
Description: fast delivery possible

ZNF287 Polyclonal Antibody, FITC Conjugated
A63850 100 µg
EUR 570.55
Description: reagents widely cited

ZNF287 Polyclonal Antibody, Biotin Conjugated
A63851 100 µg
EUR 570.55
Description: Ask the seller for details

ZNF287 ORF Vector (Human) (pORF)
ORF015038 1.0 ug DNA
EUR 354

Mouse ZNF287 shRNA Plasmid
20-abx980321
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

Zinc Finger Protein 287 (ZNF287) Antibody
abx037179-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.

Zinc Finger Protein 287 (ZNF287) Antibody
abx030175-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Zinc Finger Protein 287 (ZNF287) Antibody
abx030175-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

Zinc Finger Protein 287 (ZNF287) Antibody
20-abx013803
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.

Zinc Finger Protein 287 (ZNF287) Antibody
20-abx306051
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Zinc Finger Protein 287 (ZNF287) Antibody
20-abx324762
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

Zinc Finger Protein 287 (ZNF287) Antibody
abx330676-100ul 100 ul
EUR 425
  • Shipped within 5-10 working days.

Zinc Finger Protein 287 (ZNF287) Antibody
abx239681-100ug 100 ug
EUR 551
  • Shipped within 5-12 working days.

ZNF287 sgRNA CRISPR Lentivector set (Human)
K2697601 3 x 1.0 ug
EUR 339

Zinc Finger Protein 287 (ZNF287) Antibody (HRP)
20-abx306052
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Zinc Finger Protein 287 (ZNF287) Antibody (FITC)
20-abx306053
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Zinc Finger Protein 287 (ZNF287) Antibody (Biotin)
20-abx306054
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

ZNF287 sgRNA CRISPR Lentivector (Human) (Target 1)
K2697602 1.0 ug DNA
EUR 154

ZNF287 sgRNA CRISPR Lentivector (Human) (Target 2)
K2697603 1.0 ug DNA
EUR 154

ZNF287 sgRNA CRISPR Lentivector (Human) (Target 3)
K2697604 1.0 ug DNA
EUR 154

ZNF287 Protein Vector (Human) (pPB-C-His)
PV060149 500 ng
EUR 481

ZNF287 Protein Vector (Human) (pPB-N-His)
PV060150 500 ng
EUR 481

ZNF287 Protein Vector (Human) (pPM-C-HA)
PV060151 500 ng
EUR 481
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