July 26, 2020
Sequencing of Proteins Silver-Stained Polyacrylamide Gels
Quick, scalable technology of high-quality protein a number of sequence alignments utilizing Clustal Omega.
A number of sequence alignments are basic to many sequence evaluation strategies. Most alignments are computed utilizing the progressive alignment heuristic. These strategies are beginning to grow to be a bottleneck in some evaluation pipelines when confronted with knowledge units of the dimension of many hundreds of sequences.
Some strategies permit computation of bigger knowledge units whereas sacrificing high quality, and others produce high-quality alignments, however scale badly with the variety of sequences. On this paper, we describe a brand new program referred to as Clustal Omega, which might align just about any variety of protein sequences shortly and that delivers correct alignments. The accuracy of the package deal on smaller take a look at instances is just like that of the high-quality aligners.
On bigger knowledge units, Clustal Omega outperforms different packages when it comes to execution time and high quality. Clustal Omega additionally has highly effective options for including sequences to and exploiting data in present alignments, making use of the huge quantity of precomputed data in public databases like Pfam.
Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels.
Proteins from silver-stained gels might be digested enzymatically and the ensuing peptide analyzed and sequenced by mass spectrometry. Normal proteins yield the identical peptide maps when extracted from Coomassie- and silver-stained gels, as judged by electrospray and MALDI mass spectrometry.
Cy5 azide (non-sulfonated) |
The low nanogram vary might be reached by the protocols described right here, and the strategy is strong. A silver-stained one-dimensional gel of a fraction from yeast proteins was analyzed by nano-electrospray tandem mass spectrometry. Within the sequencing, greater than 1000 amino acids had been lined, leading to no proof of chemical modifications because of the silver staining process.
Silver staining permits a considerable shortening of pattern preparation time and will, subsequently, be preferable over Coomassie staining. This work removes a serious impediment to the low-level sequence evaluation of proteins separated on polyacrylamide gels.
Prediction of protein antigenic determinants from amino acid sequences.
A way is offered for finding protein antigenic determinants by analyzing amino acid sequences as a way to discover the purpose of biggest native hydrophilicity. That is achieved by assigning every amino acid a numerical worth (hydrophilicity worth) after which repetitively averaging these values alongside the peptide chain.
The purpose of highest native common hydrophilicity is invariably situated in, or instantly adjoining to, an antigenic determinant. It was discovered that the prediction success price trusted averaging group size, with hexapeptide averages yielding optimum outcomes.
stat-2
The tactic was developed utilizing 12 proteins for which intensive immunochemical evaluation has been carried out and subsequently was used to predict antigenic determinants for the next proteins: hepatitis B floor antigen, influenza hemagglutinins, fowl plague virus hemagglutinin, human histocompatibility antigen HLA-B7, human interferons, Escherichia coli and cholera enterotoxins, ragweed allergens Ra3 and Ra5, and streptococcal M protein.
CD8a antibody (CY5) |
The hepatitis B floor antigen sequence was synthesized by chemical means and was proven to have antigenic exercise by radioimmunoassay.
 Anti-Human HLA-DR Monoclonal Antibody PE-Cy7 Conjugated, Flow Validated |
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| FC01340-PE-Cy7 | BosterBio | 25tests, 100tests, 200tests | EUR 188 |
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Description: Anti-Human HLA-DR Monoclonal Antibody PE-Cy7 Conjugated, Flow Validated |
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 Anti-Mouse CD4 Monoclonal Antibody PE-Cyanine7 Conjugated, Flow Validated |
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| FC00344-PE-Cy7 | BosterBio | 25ug, 100ug | EUR 137 |
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Description: Rat Monoclonal Mouse CD4 Antibody PE-Cyanine7 Conjugated, Flow Validated. Validated in Flow Cytometry and tested in Mouse. |
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 Anti-Mouse CD8a Monoclonal Antibody APC-Cyanine7 Conjugated, Flow Validated |
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| FC02236-APC-Cy7 | BosterBio | 25ug, 100ug | EUR 137 |
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Description: Rat Monoclonal Mouse CD8a Antibody APC-Cyanine7 Conjugated, Flow Validated. Validated in Flow Cytometry and tested in Mouse. |
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Anti-Mouse CD4 Monoclonal Antibody PE-Cyanine7 Conjugated, Flow Validated |
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| FC00344-2-PE-Cy7 | BosterBio | 25ug, 100ug | EUR 131 |
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Description: Rat Monoclonal Mouse CD4 Antibody PE-Cyanine7 Conjugated, Flow Validated. Validated in Flow Cytometry and tested in Mouse. |
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 Monoclonal Antibody PE-Cyanine7 Conjugated, Flow Validated) Anti-Mouse Ly-6G (Gr-1) Monoclonal Antibody PE-Cyanine7 Conjugated, Flow Validated |
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| FC30404-1-PE-Cy7 | BosterBio | 25ug, 100ug | EUR 131 |
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Description: Rat Monoclonal Mouse Ly-6G (Gr-1) Antibody PE-Cyanine7 Conjugated, Flow Validated. Validated in Flow Cytometry and tested in Mouse. |
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 CY7 |
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| HY-D0825 | MedChemExpress | 10mM/1mL | EUR 506 |
 CY7-SE |
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| HY-D0824 | MedChemExpress | 25mg | EUR 394 |
 APC-Cy7 Tandem |
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| 2625 | AAT Bioquest | 1 mg | EUR 219 |
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 Cy7-PEG-NH2 |
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| abx085304-2kDa50mg | Abbexa | 2 kDa; 50 mg | EUR 954 |
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Cy7-PEG-NH2 |
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| abx085304-34kDa50mg | Abbexa | 3.4 kDa; 50 mg | EUR 954 |
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 CY7-PEG-DSPE |
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| abx085409-2kDa50mg | Abbexa | 2 kDa; 50 mg | EUR 901 |
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 Cy7 NHS ester |
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| A8109-25 | ApexBio | 25 mg | EUR 1036 |
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Description: Sulfo-Cy7 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. This reagent allows prepare Cy7-labeled biomolecules, especially useful for the labeling of delicate proteins, and proteins prone to denaturation. |
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Cy7 NHS ester |
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| A8109-5 | ApexBio | 5 mg | EUR 525 |
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Description: Sulfo-Cy7 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. This reagent allows prepare Cy7-labeled biomolecules, especially useful for the labeling of delicate proteins, and proteins prone to denaturation. |
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 Cy7-PEG-NH2,2K |
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| FL080005-2K-50mg | Biochempeg | 50mg | EUR 667 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy7-PEG-NH2,3.4K |
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| FL080005-3.4K-50mg | Biochempeg | 50mg | EUR 667 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy7-PEG-NH2,5K |
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| FL080005-5K-50mg | Biochempeg | 50mg | EUR 667 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Monoclonal Antibody (Pan-species), APC-Cy7) Fluorescein Isothiocyanate (APC-Cy7) Monoclonal Antibody (Pan-species), APC-Cy7 |
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| 4-MAO843Ge21-APC-Cy7 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Pan-species Fluorescein Isothiocyanate (APC-Cy7). This antibody is labeled with APC-Cy7. |
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 Annexin V-Cy7 conjugate |
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| 20068 | AAT Bioquest | 100 Tests | EUR 219 |
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 Cy7®-streptavidin conjugate |
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| 16914 | AAT Bioquest | 1 mg | EUR 176 |
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 RPE-Cy7-streptavidin conjugate |
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| 16916 | AAT Bioquest | 100 ug | EUR 219 |
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RPE-Cy7-streptavidin conjugate |
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| 16917 | AAT Bioquest | 1 mg | EUR 876 |
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) CD117 antibody (Allophycocyanin-CY7) |
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| 61R-CD117bMSAP5 | Fitzgerald | 100 ug | EUR 327 |
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Description: Rat monoclonal CD117 antibody (Allophycocyanin-CY7) |
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) CD11a antibody (PE-CY7) |
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| 61R-CD11aaMSPE7 | Fitzgerald | 100 ug | EUR 435 |
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Description: Rat monoclonal CD11a antibody (PE-CY7) |
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) CD16 antibody (PE-CY7) |
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| 61R-CD16gMSPEC7 | Fitzgerald | 100 ug | EUR 300 |
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Description: Rat monoclonal CD16 antibody (PE-CY7) |
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) CD19 antibody (PE-CY7) |
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| 61R-CD19aMSPEC7 | Fitzgerald | 100 ug | EUR 327 |
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Description: Rat monoclonal CD19 antibody (PE-CY7) |
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) CD19 antibody (Allophycocyanin-CY7) |
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| 61R-CD19cHUAPC7 | Fitzgerald | 100 tests | EUR 457 |
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Description: Mouse monoclonal CD19 antibody (Allophycocyanin-CY7) |
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CD19 antibody (PE-CY7) |
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| 61R-CD19cHUPEC7 | Fitzgerald | 100 tests | EUR 446 |
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Description: Mouse monoclonal CD19 antibody (PE-CY7) |
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) CD20 antibody (Allophycocyanin-CY7) |
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| 61R-CD20bHUAPC7 | Fitzgerald | 100 tests | EUR 446 |
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Description: Mouse monoclonal CD20 antibody (Allophycocyanin-CY7) |
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) CD20 antibody (PE-CY7) |
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| 61R-CD20bHUPEC7 | Fitzgerald | 100 tests | EUR 446 |
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Description: Mouse monoclonal CD20 antibody (PE-CY7) |
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) CD23 antibody (PE-CY7) |
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| 61R-CD23cMSPEC7 | Fitzgerald | 100 ug | EUR 327 |
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Description: Rat monoclonal CD23 antibody (PE-CY7) |
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) CD25 antibody (PE-CY7) |
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| 61R-CD25cMSPEC7 | Fitzgerald | 100 ug | EUR 300 |
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Description: Rat monoclonal CD25 antibody (PE-CY7) |
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CD25 antibody (PE-CY7) |
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| 61R-CD25dMSPEC7 | Fitzgerald | 100 ug | EUR 370 |
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Description: Rat monoclonal CD25 antibody (PE-CY7) |
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) CD31 antibody (PE-CY7) |
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| 61R-CD31jMSPEC7 | Fitzgerald | 100 ug | EUR 300 |
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Description: Rat monoclonal CD31 antibody (PE-CY7) |
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) CD3 antibody (Allophycocyanin-CY7) |
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| 61R-CD3gHUAPCC7 | Fitzgerald | 100 tests | EUR 446 |
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Description: Mouse monoclonal CD3 antibody (Allophycocyanin-CY7) |
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) CD3 antibody (PE-CY7) |
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| 61R-CD3gHUPEC7 | Fitzgerald | 100 tests | EUR 446 |
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Description: Mouse monoclonal CD3 antibody (PE-CY7) |
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) CD44 antibody (PE-CY7) |
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| 61R-CD44hHUPEC7 | Fitzgerald | 100 tests | EUR 446 |
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Description: Mouse monoclonal CD44 antibody (PE-CY7) |
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) CD45.1 antibody (Allophycocyanin-CY7) |
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| 61R-CD45gMSAPC7 | Fitzgerald | 100 ug | EUR 381 |
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Description: Mouse monoclonal CD45.1 antibody (Allophycocyanin-CY7) |
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) CD45.1 antibody (PE-CY7) |
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| 61R-CD45gMSPEC7 | Fitzgerald | 100 ug | EUR 300 |
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Description: Mouse monoclonal CD45.1 antibody (PE-CY7) |
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) CD45.2 antibody (PE-CY7) |
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| 61R-CD45hMSAPC7 | Fitzgerald | 100 ug | EUR 316 |
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Description: Mouse monoclonal CD45.2 antibody (PE-CY7) |
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) CD45R antibody (PE-CY7) |
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| 61R-CD45RcMSPE7 | Fitzgerald | 100 ug | EUR 300 |
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Description: Rat monoclonal CD45R antibody (PE-CY7) |
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) CD49b antibody (Allophycocyanin-CY7) |
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| 61R-CD49cMSAPC7 | Fitzgerald | 100 ug | EUR 435 |
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Description: Rat monoclonal CD49b antibody (Allophycocyanin-CY7) |
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) CD4 antibody (PE-CY7) |
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| 61R-CD4eMSPEC7 | Fitzgerald | 100 ug | EUR 300 |
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Description: Rat monoclonal CD4 antibody (PE-CY7) |
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) CD4 antibody (Allophycocyanin-CY7) |
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| 61R-CD4kHUAPCC7 | Fitzgerald | 100 tests | EUR 457 |
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Description: Mouse monoclonal CD4 antibody (Allophycocyanin-CY7) |
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CD4 antibody (PE-CY7) |
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| 61R-CD4kHUPEC7 | Fitzgerald | 100 tests | EUR 446 |
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Description: Mouse monoclonal CD4 antibody (PE-CY7) |
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) CD62L antibody (Allophycocyanin-CY7) |
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| 61R-CD62dMSAPC7 | Fitzgerald | 100 ug | EUR 381 |
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Description: Rat monoclonal CD62L antibody (Allophycocyanin-CY7) |
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) CD62L antibody (PE-CY7) |
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| 61R-CD62dMSPEC7 | Fitzgerald | 100 ug | EUR 300 |
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Description: Rat monoclonal CD62L antibody (PE-CY7) |
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) CD69 antibody (PE-CY7) |
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| 61R-CD69aMSPEC | Fitzgerald | 100 ug | EUR 300 |
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Description: Hamster monoclonal CD69 antibody (PE-CY7) |
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) CD86 antibody (PE-CY7) |
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| 61R-CD86bMSPEC7 | Fitzgerald | 100 ug | EUR 370 |
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Description: Rat monoclonal CD86 antibody (PE-CY7) |
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CD86 antibody (PE-CY7) |
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| 61R-CD86cMSPEC7 | Fitzgerald | 100 ug | EUR 403 |
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Description: Rat monoclonal CD86 antibody (PE-CY7) |
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) CD8a antibody (PE-CY7) |
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| 61R-CD8abMSPEC7 | Fitzgerald | 100 ug | EUR 300 |
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Description: Rat monoclonal CD8a antibody (PE-CY7) |
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) Cy7 azide (non-sulfonated) |
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| A8115-25 | ApexBio | 25 mg | EUR 1616 |
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Description: Cy7 azide is a labeling reagent that ready for the use in Click Chemistry labeling. This labeling dye is a near-infrared dye which has low aqueous solubility. |
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Cy7 azide (non-sulfonated) |
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| A8115-5 | ApexBio | 5 mg | EUR 583 |
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Description: Cy7 azide is a labeling reagent that ready for the use in Click Chemistry labeling. This labeling dye is a near-infrared dye which has low aqueous solubility. |
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) Cy7 maleimide (non-sulfonated) |
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| A8141-25 | ApexBio | 25 mg | EUR 525 |
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Description: Cy7 maleimide is a near-infrared, sulfhydryl reactive dye which has low aqueous solubility. This reagent can label the proteins contain free sulfhydryl groups. |
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Cy7 maleimide (non-sulfonated) |
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| A8141-5 | ApexBio | 5 mg | EUR 293 |
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Description: Cy7 maleimide is a near-infrared, sulfhydryl reactive dye which has low aqueous solubility. This reagent can label the proteins contain free sulfhydryl groups. |
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 CY7-PEG-DSPE?2K |
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| FL080096-2K-50mg | Biochempeg | 50mg | EUR 494 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Streptavidin-PE-Cy7 conjugate |
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| SV-PEC7-100 | Alpha Diagnostics | 100 tests | EUR 286 |
 Anti-EGFR PE-Cy7 |
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| T7-680-T025 | ExBio | 25 tests | EUR 154 |
Anti-EGFR PE-Cy7 |
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| T7-680-T100 | ExBio | 100 tests | EUR 268 |
 Anti-Helios PE-Cy7 |
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| T7-771-T025 | ExBio | 25 tests | EUR 154 |
Anti-Helios PE-Cy7 |
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| T7-771-T100 | ExBio | 100 tests | EUR 268 |
) Cy7 NHS ester (non-sulfonated) |
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| A8104-25 | ApexBio | 25 mg | EUR 525 |
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Description: Cy7 NHS ester, also called Cy7 monofunctional N-hydroxysuccinimide ester or Cy7-NHS [1], is a near-infrared fluorescent [2] and soluble sulfonated cyanine dye [3] with a molecular weight of 828 Da[2], able to be dissolved in DMSO [1] and ready for conjugation to proteins [4]. |
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Cy7 NHS ester (non-sulfonated) |
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| A8104-5 | ApexBio | 5 mg | EUR 293 |
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Description: Cy7 NHS ester, also called Cy7 monofunctional N-hydroxysuccinimide ester or Cy7-NHS [1], is a near-infrared fluorescent [2] and soluble sulfonated cyanine dye [3] with a molecular weight of 828 Da[2], able to be dissolved in DMSO [1] and ready for conjugation to proteins [4]. |
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Cy7 NHS ester (non-sulfonated) |
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| A8104-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 583 |
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Description: Cy7 NHS ester, also called Cy7 monofunctional N-hydroxysuccinimide ester or Cy7-NHS [1], is a near-infrared fluorescent [2] and soluble sulfonated cyanine dye [3] with a molecular weight of 828 Da[2], able to be dissolved in DMSO [1] and ready for conjugation to proteins [4]. |
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) Cy7 carboxylic acid (non-sulfonated) |
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| A8135-25 | ApexBio | 25 mg | EUR 525 |
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Description: Cy7 carboxylic acid is a near-infrared fluorescent dye which has low aqueous solubility. As for coupling and labeling reactions, pre-activated Cy7 NHS ester or water-soluble sulfo-Cy7 NHS ester would be considered. |
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Cy7 carboxylic acid (non-sulfonated) |
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| A8135-5 | ApexBio | 5 mg | EUR 293 |
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Description: Cy7 carboxylic acid is a near-infrared fluorescent dye which has low aqueous solubility. As for coupling and labeling reactions, pre-activated Cy7 NHS ester or water-soluble sulfo-Cy7 NHS ester would be considered. |
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 Anti-Hu CD45 APC-Cy7 |
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| T4-160-T100 | ExBio | 100 tests | EUR 268 |
 Anti-Hu CD49d APC-Cy7 |
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| T4-171-T100 | ExBio | 100 tests | EUR 268 |
 Anti-Hu CD3 APC-Cy7 |
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| T4-173-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD3 APC-Cy7 |
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| T4-173-T100 | ExBio | 100 tests | EUR 268 |
 Anti-Hu CD8 APC-Cy7 |
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| T4-207-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD8 APC-Cy7 |
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| T4-207-T100 | ExBio | 100 tests | EUR 268 |
 Anti-Hu CD9 APC-Cy7 |
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| T4-208-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD9 APC-Cy7 |
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| T4-208-T100 | ExBio | 100 tests | EUR 268 |
 Anti-Hu CD10 APC-Cy7 |
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| T4-209-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD10 APC-Cy7 |
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| T4-209-T100 | ExBio | 100 tests | EUR 268 |
 Anti-Hu CD25 APC-Cy7 |
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| T4-218-T100 | ExBio | 100 tests | EUR 268 |
 Anti-Hu CD43 APC-Cy7 |
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| T4-220-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD43 APC-Cy7 |
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| T4-220-T100 | ExBio | 100 tests | EUR 268 |
Anti-Hu CD45 APC-Cy7 |
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| T4-222-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD45 APC-Cy7 |
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| T4-222-T100 | ExBio | 100 tests | EUR 268 |
 Anti-Hu CD71 APC-Cy7 |
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| T4-235-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD71 APC-Cy7 |
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| T4-235-T100 | ExBio | 100 tests | EUR 268 |
 Anti-Hu CD14 APC-Cy7 |
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| T4-293-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD14 APC-Cy7 |
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| T4-293-T100 | ExBio | 100 tests | EUR 268 |
 Anti-Hu CD19 APC-Cy7 |
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| T4-305-T025 | ExBio | 25 tests | EUR 154 |
Anti-Hu CD19 APC-Cy7 |
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| T4-305-T100 | ExBio | 100 tests | EUR 268 |
 Anti-Hu CD27 APC-Cy7 |
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| T4-308-T100 | ExBio | 100 tests | EUR 268 |
 Anti-Hu CD4 APC-Cy7 |
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| T4-359-T025 | ExBio | 25 tests | EUR 154 |
Extending the therapy of spine energetics in protein drive fields: limitations of gas-phase quantum mechanics in reproducing protein conformational distributions in molecular dynamics simulations
Computational research of proteins based mostly on empirical drive fields symbolize a robust instrument to acquire structure-function relationships at an atomic stage, and are central in present efforts to resolve the protein folding downside. The outcomes from research making use of these instruments are, nonetheless, depending on the standard of the drive fields used. Specifically, correct therapy of the peptide spine is essential to realize consultant conformational distributions in simulation research.
To enhance the therapy of the peptide spine, quantum mechanical (QM) and molecular mechanical (MM) calculations had been undertaken on the alanine, glycine, and proline dipeptides, and the outcomes from these calculations had been mixed with molecular dynamics (MD) simulations of proteins in crystal and aqueous environments. QM potential vitality maps of the alanine and glycine dipeptides on the LMP2/cc-pVxZ//MP2/6-31G* ranges, the place x = D, T, and Q, had been decided, and are in comparison with accessible QM research on these molecules.
The LMP2/cc-pVQZ//MP2/6-31G* vitality surfaces for all three dipeptides had been then used to enhance the MM therapy of the dipeptides. These enhancements included further parameter optimization by way of Monte Carlo simulated annealing and extension of the potential vitality perform to comprise peptide spine phi, psi dihedral crossterms or a phi, psi grid-based vitality correction time period.
Concurrently, MD simulations of as much as seven proteins of their crystalline environments had been used to validate the drive area enhancements. Comparability with QM and crystallographic knowledge confirmed that an extra optimization of the phi, psi dihedral parameters together with the grid-based vitality correction had been required to yield vital enhancements over the CHARMM22 drive area.
Fluorescein Isothiocyanate (APC-Cy7) Monoclonal Antibody (Pan-species), APC-Cy7 |
Nevertheless, systematic deviations within the therapy of phi and psi within the helical and sheet areas had been evident. Accordingly, empirical changes had been made to the grid-based vitality correction for alanine and glycine to account for these systematic variations.
These changes result in higher deviations from QM knowledge for the 2 dipeptides but additionally yielded improved settlement with experimental crystallographic knowledge. These enhancements improve the standard of the CHARMM drive area in treating proteins. This extension of the potential vitality perform is anticipated to facilitate improved therapy of organic macromolecules by way of MM approaches in general.
 Cy5 tertrazine [Cy5 tertrazine] |
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| 911 | AAT Bioquest | 1 mg | EUR 219 |
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 CY5 |
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| HY-D0821 | MedChemExpress | 25mg | EUR 1496 |
) PE-Cy5 (Phycoerhthrin-Cy5 Conjugate) |
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| 7850-250 | Biovision | EUR 370 | |
 CY5-SE |
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| HY-D0819 | MedChemExpress | 25mg | EUR 463 |
 CY5-YNE |
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| HY-D0820 | MedChemExpress | 10mM/1mL | EUR 786 |
 CY5-N3 |
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| HY-D0832 | MedChemExpress | 5mg | EUR 533 |
 DBCO-Cy5 |
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| 923 | AAT Bioquest | 1 mg | EUR 132 |
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 Cy5 azide |
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| A8128-25 | ApexBio | 25 mg | EUR 1732 |
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Description: Sulfo-Cy5 azide is a sulfonated, hydrophilic and highly water soluble dye for Click chemistry. Due to this features, the dye can be used for labeling biomolecules, especially useful for the labeling of delicate proteins and proteins prone to denaturation, purely aqueous conditions. |
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Cy5 azide |
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| A8128-5 | ApexBio | 5 mg | EUR 618 |
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Description: Sulfo-Cy5 azide is a sulfonated, hydrophilic and highly water soluble dye for Click chemistry. Due to this features, the dye can be used for labeling biomolecules, especially useful for the labeling of delicate proteins and proteins prone to denaturation, purely aqueous conditions. |
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 Cy5 phosphoramidite |
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| 6071 | AAT Bioquest | 100 umoles | EUR 50 |
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 Mouse anti-Human CD3, PE-Cy5 Conjugated mAb |
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| 28006-50Tests | SAB | 50 Tests | EUR 253 |
 Mouse anti-Human CD4, PE-Cy5 Conjugated mAb |
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| 28013-50Tests | SAB | 50 Tests | EUR 253 |
 Mouse anti-Human CD8, PE-Cy5 Conjugated mAb |
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| 28028-50Tests | SAB | 50 Tests | EUR 253 |
 Mouse anti-Human CD14, PE-Cy5 Conjugated mAb |
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| 28053-50Tests | SAB | 50 Tests | EUR 252 |
 Mouse anti-Human CD19, PE-Cy5 Conjugated mAb |
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| 28074-50Tests | SAB | 50 Tests | EUR 252 |
 Mouse anti-Human CD45, PE-Cy5 Conjugated mAb |
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| 28144-50Tests | SAB | 50 Tests | EUR 253 |
 Cy5-PEG-SH |
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| abx085228-1kDa50mg | Abbexa | 1 kDa; 50 mg | EUR 871 |
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Cy5-PEG-SH |
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| abx085228-2kDa50mg | Abbexa | 2 kDa; 50 mg | EUR 913 |
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Cy5-PEG-SH |
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| abx085228-34kDa50mg | Abbexa | 3.4 kDa; 50 mg | EUR 940 |
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Cy5-PEG-SH |
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| abx085228-5kDa50mg | Abbexa | 5 kDa; 50 mg | EUR 940 |
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 Cy5-PEG-COOH |
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| abx085357-10kDa50mg | Abbexa | 10 kDa; 50 mg | EUR 499 |
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Cy5-PEG-COOH |
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| abx085357-20kDa50mg | Abbexa | 20 kDa; 50 mg | EUR 499 |
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Cy5-PEG-COOH |
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| abx085357-2kDa50mg | Abbexa | 2 kDa; 50 mg | EUR 535 |
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Cy5-PEG-COOH |
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| abx085357-5kDa50mg | Abbexa | 5 kDa; 50 mg | EUR 535 |
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 Cy5-PEG-Mal |
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| abx085358-10kDa50mg | Abbexa | 10 kDa; 50 mg | EUR 546 |
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Cy5-PEG-Mal |
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| abx085358-1kDa50mg | Abbexa | 1 kDa; 50 mg | EUR 546 |
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Cy5-PEG-Mal |
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| abx085358-2kDa50mg | Abbexa | 2 kDa; 50 mg | EUR 570 |
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 Glucagon-Cy5 Peptide |
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| abx267068-1mg | Abbexa | 1 mg | EUR 2068 |
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 Cy5 NHS ester |
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| A8108-25 | ApexBio | 25 mg | EUR 746 |
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Description: Sulfo-Cy5 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. For the labeling of various amine-containing molecules in aqueous phase, no organic co-solvent is needed. |
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Cy5 NHS ester |
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| A8108-5 | ApexBio | 5 mg | EUR 293 |
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Description: Sulfo-Cy5 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. For the labeling of various amine-containing molecules in aqueous phase, no organic co-solvent is needed. |
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 Cy5 carboxylic acid |
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| A8137-25 | ApexBio | 25 mg | EUR 1036 |
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Description: Sulfo-Cy5 carboxylic acid is a non-activated, sulfonated, hydrophilic and highly water soluble dye. Non-Sulfo-Cy5 carboxylic acid analog is available. For kinds of researches related experiments, using pre-activated sulfo-Cy5 NHS ester is preferred |
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Cy5 carboxylic acid |
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| A8137-5 | ApexBio | 5 mg | EUR 525 |
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Description: Sulfo-Cy5 carboxylic acid is a non-activated, sulfonated, hydrophilic and highly water soluble dye. Non-Sulfo-Cy5 carboxylic acid analog is available. For kinds of researches related experiments, using pre-activated sulfo-Cy5 NHS ester is preferred |
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 Azido-Cy5 tyramide |
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| 11061 | AAT Bioquest | 1 mg | EUR 306 |
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 PE-Cy5 Tandem |
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| 2610 | AAT Bioquest | 1 mg | EUR 219 |
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) CD117 antibody (CY5) |
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| 61R-CD117bMSCY5 | Fitzgerald | 100 ug | EUR 413 |
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Description: Rat monoclonal CD117 antibody (CY5) |
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) CD11b antibody (CY5) |
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| 61R-CD11baMSCY5 | Fitzgerald | 100 ug | EUR 413 |
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Description: Rat monoclonal CD11b antibody (CY5) |
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) CD19 antibody (CY5) |
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| 61R-CD19aMSCY5 | Fitzgerald | 100 ug | EUR 435 |
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Description: Rat monoclonal CD19 antibody (CY5) |
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CD19 antibody (CY5) |
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| 61R-CD19cHUCY5 | Fitzgerald | 100 tests | EUR 435 |
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Description: Mouse monoclonal CD19 antibody (CY5) |
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) CD3e antibody (CY5) |
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| 61R-CD3egMSCY | Fitzgerald | 100 ug | EUR 284 |
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Description: Hamster monoclonal CD3e antibody (CY5) |
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CD3e antibody (CY5) |
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| 61R-CD3ehMSCY | Fitzgerald | 100 ug | EUR 284 |
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Description: Rat monoclonal CD3e antibody (CY5) |
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) CD3 antibody (CY5) |
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| 61R-CD3gHUCY5 | Fitzgerald | 100 tests | EUR 446 |
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Description: Mouse monoclonal CD3 antibody (CY5) |
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) CD45.1 antibody (CY5) |
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| 61R-CD45gMSCY5 | Fitzgerald | 100 ug | EUR 457 |
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Description: Mouse monoclonal CD45.1 antibody (CY5) |
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) CD45.2 antibody (CY5) |
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| 61R-CD45hMSCY5 | Fitzgerald | 100 ug | EUR 381 |
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Description: Mouse monoclonal CD45.2 antibody (CY5) |
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) CD45R antibody (CY5) |
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| 61R-CD45RcMSCY5 | Fitzgerald | 100 ug | EUR 413 |
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Description: Rat monoclonal CD45R antibody (CY5) |
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) CD4 antibody (CY5) |
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| 61R-CD4eMSCY | Fitzgerald | 100 ug | EUR 403 |
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Description: Rat monoclonal CD4 antibody (CY5) |
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CD4 antibody (CY5) |
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| 61R-CD4kHUCY5 | Fitzgerald | 100 tests | EUR 435 |
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Description: Mouse monoclonal CD4 antibody (CY5) |
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) CD8a antibody (CY5) |
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| 61R-CD8abMSCY5 | Fitzgerald | 100 ug | EUR 413 |
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Description: Rat monoclonal CD8a antibody (CY5) |
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CD8a antibody (CY5) |
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| 61R-CD8adCKCY5 | Fitzgerald | 100 ug | EUR 543 |
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Description: Mouse monoclonal CD8a antibody (CY5) |
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CD8a antibody (CY5) |
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| 61R-CD8aeCKCY5 | Fitzgerald | 100 ug | EUR 457 |
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Description: Mouse monoclonal CD8a antibody (CY5) |
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 PE-Cy5 Calibration Kit |
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| ECFP-F4-5K | Spherotech | 5X1 mL | EUR 310 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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 Cy5-PEG-SH,10K |
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| FL078003-10K-50mg | Biochempeg | 50mg | EUR 544 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-SH,1K |
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| FL078003-1K-50mg | Biochempeg | 50mg | EUR 605 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-SH,2K |
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| FL078003-2K-50mg | Biochempeg | 50mg | EUR 568 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-SH,3.4K |
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| FL078003-3.4K-50mg | Biochempeg | 50mg | EUR 544 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-SH,5K |
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| FL078003-5K-50mg | Biochempeg | 50mg | EUR 544 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-COOH,10K |
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| FL078017-10K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-COOH,1K |
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| FL078017-1K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-COOH,20K |
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| FL078017-20K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-COOH,2K |
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| FL078017-2K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-COOH,3.4K |
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| FL078017-3.4K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-COOH,5K |
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| FL078017-5K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-Mal,10K |
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| FL078022-10K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-Mal,1K |
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| FL078022-1K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-Mal,20K |
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| FL078022-20K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-Mal,2K |
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| FL078022-2K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-Mal,3.4K |
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| FL078022-3.4K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-Mal,5K |
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| FL078022-5K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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) CD45RO Antibody (PE-Cy5) |
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| abx134008-100Assays | Abbexa | 100 Assays | EUR 495 |
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CD45RO Antibody (PE-Cy5) |
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| abx134008-50Assays | Abbexa | 50 Assays | EUR 398 |
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) Cy5 NHS ester(Et) |
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| A8769-25 | ApexBio | 25 mg | EUR 525 |
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Cy5 NHS ester(Et) |
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| A8769-5 | ApexBio | 5 mg | EUR 293 |
 Cy5 Bis NHS ester |
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| A8773-25 | ApexBio | 25 mg | EUR 525 |
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Cy5 Bis NHS ester |
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| A8773-5 | ApexBio | 5 mg | EUR 293 |
 Cy5 Bis carboxylic acid |
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| A8775-25 | ApexBio | 25 mg | EUR 525 |
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Cy5 Bis carboxylic acid |
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| A8775-5 | ApexBio | 5 mg | EUR 293 |
) Cy5 carboxylic acid(Et) |
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| A8777-25 | ApexBio | 25 mg | EUR 525 |
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Cy5 carboxylic acid(Et) |
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| A8777-5 | ApexBio | 5 mg | EUR 293 |
) Cy5 azide (non-sulfonated) |
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| A8113-25 | ApexBio | 25 mg | EUR 1616 |
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Description: Cy5 azide is a labeling reagent ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. |
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Cy5 azide (non-sulfonated) |
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| A8113-5 | ApexBio | 5 mg | EUR 583 |
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Description: Cy5 azide is a labeling reagent ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. |
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) Cy5 alkyne (non-sulfonated) |
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| A8131-25 | ApexBio | 25 mg | EUR 1616 |
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Description: Cy5 alkyne, with low aqueous solubility is a dye ready for the use in Click Chemistry reaction. Various molecules via Click Chemistry reaction can be attached by this deeply colored, and photostable Cy5 fluorophore. |
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Cy5 alkyne (non-sulfonated) |
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| A8131-5 | ApexBio | 5 mg | EUR 583 |
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Description: Cy5 alkyne, with low aqueous solubility is a dye ready for the use in Click Chemistry reaction. Various molecules via Click Chemistry reaction can be attached by this deeply colored, and photostable Cy5 fluorophore. |
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) Cy5 maleimide (non-sulfonated) |
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| A8139-25 | ApexBio | 25 mg | EUR 525 |
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Description: Cy5 maleimide is a mono-reactive dye for selectively labeling peptides and proteins which couples with thiol groups. |
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Cy5 maleimide (non-sulfonated) |
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| A8139-5 | ApexBio | 5 mg | EUR 293 |
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Description: Cy5 maleimide is a mono-reactive dye for selectively labeling peptides and proteins which couples with thiol groups. |
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) Cy5 amine (non-sulfonated) |
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| A8143-25 | ApexBio | 25 mg | EUR 525 |
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Description: Cy5 amine is a reactive dye which contains amino group. This dye can be coupled with a variety of activated esters and other electrophilic reagents. |
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Cy5 amine (non-sulfonated) |
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| A8143-5 | ApexBio | 5 mg | EUR 293 |
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Description: Cy5 amine is a reactive dye which contains amino group. This dye can be coupled with a variety of activated esters and other electrophilic reagents. |
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) Cy5 hydrazide (non-sulfonated) |
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| A8145-25 | ApexBio | 25 mg | EUR 525 |
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Description: Cy5 hydrazide is a reactive dye which reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. The labeling regent is used for the labeling of aldehydes and ketones. |
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Cy5 hydrazide (non-sulfonated) |
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| A8145-5 | ApexBio | 5 mg | EUR 293 |
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Description: Cy5 hydrazide is a reactive dye which reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. The labeling regent is used for the labeling of aldehydes and ketones. |
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